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BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine cusbo CAS:6578-06-9
BCIP-Toluidine, oo sidoo kale loo yaqaan cusbada 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo cilmi-baarista bayoolajiga molecular-ka iyo bayochemistry-ga. Waxaa ugu horreyn loo isticmaalaa substrate-ka baaritaanka immunosorbent-ka ee enzyme-linked (ELISAs) ama farsamooyinka rinjiyeynta immunohistochemical si loo ogaado dhaqdhaqaaqa phosphatase alkaline.
Marka BCIP-Toluidine lagu kala jaro fosfatase alkaline, waxay martaa falgal isbeddel midab ah, taasoo soo saarta daadasho buluug-guduud ah. Falcelintani waxay u oggolaanaysaa muuqaalka iyo cabbirka dhaqdhaqaaqa fosfatase alkaline ee muunadaha. Xoogga midabka waxaa lagu cabbiri karaa si sawir-qaade ah waxayna si toos ah ugu dhigantaa dhaqdhaqaaqa ensaymka.
Isticmaalka BCIP-Toluidine oo ah substrate gaar ahaan waa wax caadi ah marka la adeegsanayo calaamadaynta DNA ama RNA, sida in situ hybridization (ISH) ama Southern and Northern blotting. Waxay bixisaa hab lagu kalsoonaan karo oo xasaasi ah oo lagu ogaanayo jiritaanka asiidhyada nucleic-ga ee bartilmaameedka ah ee muunadaha.
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3-Aminophthalhydrazide CAS:521-31-3
3-Aminophthalhydrazide waa isku-dhis kiimiko ah oo leh qaacidada molecular-ka C8H8N2O. Waxaa loogu yeeraa aminohydrazide oo ka soo jeeda phthalic acid. Isku-darkani wuxuu ka kooban yahay laf-dhabar phthalic acid ah oo leh laba atam oo haydarojiin ah oo lagu beddelay koox amino ah iyo koox hydrazine ah.
3-Aminophthalhydrazide waxay leedahay adeegsiyo kala duwan oo ku saabsan kiimikada dabiiciga ah iyo daawada. Waxaa loo isticmaali karaa sidii agab bilow ah oo lagu sameeyo isku-darka daawooyinka kala duwan iyo midabaynta. Intaa waxaa dheer, waxaa badanaa loo isticmaalaa diyaarinta saldhigyada Schiff, kuwaas oo ah dhexdhexaad muhiim ah oo ku jira isku-darka dabiiciga ah.
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4-Aminoantipyrine CAS:83-07-8
4-Aminoantipyrine, oo sidoo kale loo yaqaan aminoantipyrine ama aminopyrine, waa isku-dhis dabiici ah oo macmal ah. Waxaa badanaa loo isticmaalaa sidii reagent-ka kiimikada falanqaynta iyo warshadaha dawooyinka. Qaab-dhismeedkeeda kiimikada wuxuu ka kooban yahay giraan udgoon oo leh koox amino (NH2) iyo koox pyrazolone ah, taasoo ka dhigaysa isku-dhis badan oo loogu talagalay codsiyada kala duwan.
Kiimikada falanqaynta, 4-Aminoantipyrine waxaa si weyn loogu isticmaalaa sidii falgal koromosoom ah oo ku jira tijaabooyinka midabka. Waxay la falgashaa walxo gaar ah, sida phenols iyo peroxides, si ay u sameeyaan isku-dhafan midab leh oo lagu cabbiri karo si sawir-qaade ah. Sifadan waxay ka dhigaysaa mid waxtar leh baaritaanno kala duwan, oo ay ku jiraan go'aaminta ensaymada sida peroxidase, glucose, iyo amylase.
Warshadaha dawooyinka, 4-Aminoantipyrine waxaa taariikh ahaan loo isticmaali jiray daawo xanuun baabi'iye iyo qandho jabis ah. .
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TAPS-NA CAS:91000-53-2 Qiimaha Soo-saaraha
Cusbada sodium ee N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid, oo sidoo kale loo yaqaan TAPS-Na, waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo sidii wakiil kaydin ah oo loogu talagalay codsiyada bayoolojiga iyo bayoolaji ee kala duwan. Waa isku-dhis zwitterionic ah, taasoo la macno ah inay leedahay labadaba dallacaad togan iyo taban, taasoo ka caawisa ilaalinta pH deggan oo ku jirta xalalka.
TAPS-Na waxay leedahay kala duwanaansho pH ah oo u dhaxaysa 7.7 ilaa 9.1, taasoo ka dhigaysa mid ku habboon codsiyada u baahan jawi pH ah oo alkaline ah. Badanaa waxaa loo isticmaalaa cilmi-baarista borotiinka iyo ensaymka, iyo sidoo kale farsamooyinka bayoolajiga molecular-ka sida electrophoresis-ka jel iyo taxanaha DNA-da.
Sida cusbo soodhiyam ah, TAPS-Na aad ayuu ugu milmaa biyaha, taasoo u oggolaanaysa diyaarinta fudud ee xalalka la keydiyay. Waxaa loo yaqaanaa sunta yar iyo faragelinta yar ee molecules-ka bayoolojiga, taasoo ka dhigaysa doorasho la doorbido tijaabooyin badan.
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5,5-Dithiobis(2-nitrobenzoic acid) CAS:69-78-3
5,5-Dithiobis (2-nitrobenzoic acid), oo sidoo kale loo yaqaan DTNB ama reagent Ellman, waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo cilmi-baarista kiimikada iyo dawooyinka. Waa budo huruud ah oo crystalline ah oo si aad ah ugu milma biyaha iyo dareerayaasha dabiiciga ah.
DTNB waxaa ugu horreyn loo isticmaalaa in lagu cabbiro isku-darka kooxaha sulfhydryl (-SH) ee borotiinnada iyo peptides-ka. Isku-darka wuxuu la falgalaa thiols, taasoo keenta sameynta disulfide isku dhafan oo sii daaya 2-nitro-5-thiobenzoate, kaas oo leh midab jaalle ah oo lagu cabbiri karo spectrophotometric. Marka la cabbiro xoogga midabka jaallaha ah, waxaa suurtagal ah in la qiyaaso isku-darka thiols ee muunad. Farsamadan waxaa badanaa loo isticmaalaa in lagu barto isdhexgalka borotiinka-borotiinka, laabashada borotiinka, iyo falcelinta ensaymikada ee ku lug leh kooxaha thiol.
Marka laga soo tago qiyaasta thiol, DTNB waxaa loo adeegsaday codsiyada kale, sida qiimeynta xaaladda redox ee unugyada, barashada xasilloonida isku-dhafka birta-sulfur, iyo cabbiraadda dhaqdhaqaaqa enzymes-ka kala duwan.
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PHENOLPHTHALEIN MONOPHOSPHATE BIS(CYCLOHEXYLAMMONIUM) CUSBO CAS:14815-59-9
Cusbada Phenolphthalein monophosphate dicyclohexylammonium waa isku-dhis kiimiko ah oo laga soo qaatay phenolphthalein, oo ah tilmaame pH ah oo si caadi ah loogu isticmaalo goobaha shaybaarka. Qaabka cusbada dicyclohexylammonium ee phenolphthalein monophosphate waxaa badanaa loo isticmaalaa sidii falanqayn falanqayn ah oo ku saabsan cilmi-baarista bayoolaji iyo molecular bayooloji. Waxaa ugu horreyn loo isticmaalaa tijaabooyinka ama tijaabooyinka si loo ogaado oo loo cabbiro jiritaanka ensaymyo gaar ah, oo ay ku jiraan kuwa ku lug leh hawlaha ATP (adenosine triphosphate) hydrolysis ama phosphatase. Sifooyinkeeda gaarka ah waxay ka dhigayaan qalab waxtar leh baaritaannada sayniska ee la xiriira kinetics ensaym iyo hababka unugyada..
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NSP-SA-NHS CAS:199293-83-9 Qiimaha Soo-saaraha
NSP-SA-NHS waa isku-dhis ka kooban N-succinimidyl-6-(3′-pyridyldithio)hexanoate (NSP) iyo N-hydroxysuccinimide (NHS). Waxaa caadi ahaan loo isticmaalaa sidii wakiil isku-xidha falgallada bayoolojiga, gaar ahaan isku-xidhka peptides-ka, borotiinnada, ama molecules-ka kale ee ka kooban amine-ka dusha sare ama molecules-ka kale. Qaybta NSP waxay suurtogal ka dhigaysaa sameynta xiriir thioester oo deggan oo leh molecule-ka bartilmaameedka ah, halka qaybta NHS ay u oggolaanayso falcelin dambe oo lala sameeyo amine-yada aasaasiga ah. NSP-SA-NHS si weyn ayaa loogu isticmaalaa cilmi-baaris iyo codsiyo kala duwan oo bayooloji ah, oo ay ku jiraan diyaarinta baaritaannada difaaca jirka, calaamadaynta antibody-ga, daraasadaha isdhexgalka borotiinka-borotiinka, iyo joojinta borotiinka.
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N-(1-Naphthyl)-3-aminopropanesulfonic Asiidh Sodium Cusbo CAS:104484-71-1
Tilmaamaha pH: Isku-dhafkan wuxuu muujiyaa isbeddellada midabka ee ku xiran pH, taasoo ka dhigaysa tilmaame pH oo waxtar leh baaritaanno iyo tijaabooyin kala duwan. Waxaa loo isticmaali karaa in lagu kormeero isbeddellada pH ee muunadaha bayoolojiga, falcelinta kiimikada, iyo nidaamyada kale.
Baaritaanka Iftiinka: Cusbada sodium ee N-(1-Naphthyl)-3-aminopropanesulfonic acid waxay leedahay sifooyin iftiin ah oo ku dhex jira, taasoo ka dhigaysa mid ku habboon daraasadaha ku salaysan iftiinka iyo codsiyada calaamadaynta. Waxaa lagu dari karaa farsamooyinka falanqaynta si loo arko loona la socdo molecules-ka bayoolojiga ama qaab-dhismeedka unugyada.
Baaritaannada Ensaymka: Isku-darkaan waxaa badanaa loo isticmaalaa sidii substrate ama inhibitor ah baaritaannada ensaymka, gaar ahaan kuwa ku lug leh kooxaha sulfonate ama dhaqdhaqaaqa ensaymka ee ay saameeyeen isbeddellada pH. Waxay ka caawin kartaa cilmi-baarayaasha inay bartaan kinetics-ka ensaymka, farsamooyinka joojinta, iyo isdhexgalka ensaymka-substrate.
Qaybta Kaydka: Cusbada soodhiyamka ee N-(1-Naphthyl)-3-aminopropanesulfonic acid waxaa loo isticmaali karaa qayb ka mid ah nidaamyada kaydka gaarka ah, gaar ahaan kuwa u baahan koox aashitada sulfonic ama heerarka pH qaarkood. Waxay gacan ka geysataa ilaalinta xaaladaha pH ee ugu wanaagsan ee dejinta tijaabada.
Isku-darka Kiimikada: Sababtoo ah qaab-dhismeedkeeda kiimikada ee gaarka ah, milixda sodium ee N-(1-Naphthyl)-3-aminopropanesulfonic acid waxay u adeegi kartaa sidii baloog dhisme ama walxo bilow ah oo lagu sameeyo isku-darka molecules-ka dabiiciga ah ama isku-dhafka dawooyinka.
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Aashitada aashitada N-(2-Acetamide) aashitada iminodiacetic CAS:26239-55-4
Aashitada N-(2-Acetamido)iminodiacetic waa isku-dhis ka tirsan fasalka amino acids-ka loo yaqaan wakiilada chelating. Waxaa caadi ahaan loo isticmaalaa sidii wakiil isku-dhafan, gaar ahaan codsiyada caafimaadka iyo warshadaha. Isku-dhisku wuxuu leeyahay sifooyin bir ah oo aad u fiican, taasoo la macno ah inuu samayn karo isku-dhisyo deggan oo leh ion bir ah oo kala duwan isagoo isku dubaridaya iyaga oo u maraya kooxo shaqeynaya..
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Disodium 4-nitrophenylphosphate CAS:4264-83-9
Disodium 4-nitrophenylphosphate waa isku-dhis kiimiko ah oo leh qaacidada molecular-ka C6H4NO6PNa2. Waa budo crystalline ah oo fiican oo caadiyan loo isticmaalo cilmi-baarista bayoolojiga iyo codsiyada bayoolojiga molecular-ka. Badanaa waxaa loo isticmaalaa sidii substrate-ka baaritaanka ensaymka, gaar ahaan ensaymada phosphatase. Isku-darkaan waxaa loo yaqaanaa inuu mariyo hydrolysis-ka xalalka biyaha, taasoo keenta sii deynta ion-ka fosfate-ka ee bilaashka ah.
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6-methoxyquinoline CAS:5263-87-6
6-Methoxyquinoline waa iskudhis dabiici ah oo leh qaacidada molecular-ka C10H9NO. Waa iskudhis udgoon oo heterocyclic ah oo ka kooban giraan quinoline ah oo lagu daray kooxda methoxy ee booska 6-aad.
Isku-darkani wuxuu leeyahay codsiyo kala duwan oo ku saabsan isku-darka dabiiciga ah iyo kiimikada daawada. Waxay u adeegi kartaa sidii baloog dhisme oo loogu talagalay isku-darka waxyaabaha kale ee laga soo saaro quinoline iyo isku-darka la xiriira.
Kiimikada daawada, 6-Methoxyquinoline waxay muujisay waxqabadyo farsamo oo suurtagal ah. Waxaa lagu bartay sifooyinka jeermiska dila, ka hortagga duumada, iyo ka hortagga kansarka..
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4-Benzylamino-7-nitrobenz-2-oxa-1,3-diazole CAS:18378-20-6
4-Benzylamino-7-nitrobenz-2-oxa-1,3-diazole waa isku-dhis kiimiko ah oo leh qaacido molecular ah oo C14H11N5O3 ah. Waa isku-dhis heterocyclic ah oo ka kooban giraan diazole ah oo lagu daray giraan benzene ah. Isku-darkani waxaa loo yaqaanaa sifooyinkiisa fluorescent-ka waxaana badanaa loo isticmaalaa midab fluorescent ah cilmi-baarista bayoolojiga iyo codsiyada sawir-qaadista. Kooxda benzylamino ee ku xiran giraan diazole waxay xoojisaa milmideeda iyo xasilloonideeda. Kooxda nitro iyo atamka oxa (oksijiin) ee giraan diazole waxay gacan ka geystaan sifooyinka kiimikada iyo jireed ee gaarka ah.
