5,5-Dithiobis(2-nitrobenzoic acid) CAS:69-78-3

5,5-Dithiobis (2-nitrobenzoic acid), oo sidoo kale loo yaqaan DTNB ama reagent Ellman, waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo cilmi-baarista kiimikada iyo dawooyinka. Waa budo huruud ah oo crystalline ah oo si aad ah ugu milma biyaha iyo dareerayaasha dabiiciga ah.

DTNB waxaa ugu horreyn loo isticmaalaa in lagu cabbiro isku-darka kooxaha sulfhydryl (-SH) ee borotiinnada iyo peptides-ka. Isku-darka wuxuu la falgalaa thiols, taasoo keenta sameynta disulfide isku dhafan oo sii daaya 2-nitro-5-thiobenzoate, kaas oo leh midab jaalle ah oo lagu cabbiri karo spectrophotometric. Marka la cabbiro xoogga midabka jaallaha ah, waxaa suurtagal ah in la qiyaaso isku-darka thiols ee muunad. Farsamadan waxaa badanaa loo isticmaalaa in lagu barto isdhexgalka borotiinka-borotiinka, laabashada borotiinka, iyo falcelinta ensaymikada ee ku lug leh kooxaha thiol.

Marka laga soo tago qiyaasta thiol, DTNB waxaa loo adeegsaday codsiyada kale, sida qiimeynta xaaladda redox ee unugyada, barashada xasilloonida isku-dhafka birta-sulfur, iyo cabbiraadda dhaqdhaqaaqa enzymes-ka kala duwan.

Dalbo ama Codso Qiimayn

Faahfaahinta Badeecada

Calaamadaha Alaabta

Codsiga iyo Saamaynta

Tirakoobka Thiol: DTNB waxaa badanaa loo isticmaalaa in lagu qiimeeyo heerka thiols ee muunadaha borotiinka. Macluumaadkani waa muhiim si loo barto isdhexgalka borotiinka-borotiinka, laablaabka borotiinka, iyo falcelinta enzymatic ee ku lug leh kooxaha thiol. Jawaabta midabka ee DTNB waxay u oggolaanaysaa in la qiyaaso thiols iyada oo loo marayo cabbiraadaha spectrophotometric.

Qiimaynta Gobolka Redox: DTNB waxaa loo adeegsaday in lagu qiimeeyo xaaladda redox ee unugyada, unugyada, ama muunadaha bayoolojiga. Iyagoo cabbiraya isbeddellada ku yimaada heerka thiol, cilmi-baarayaashu waxay heli karaan aragtiyo ku saabsan walbahaarka oksaydhka, dhaqdhaqaaqa antioxidant-ka, iyo waddooyinka calaamadaynta redox.

Baaritaannada Ensaym-ka: Falcelinta gaarka ah ee DTNB oo leh thiols waxay ka dhigaysaa qalab qiimo leh oo loogu talagalay baaritaannada ensaym-ka. Ensaymyo badan, sida glutathione-S-transferases, cystathionine-γ-lyase, iyo thioredoxin reductase, waxay ku xiran yihiin thiol waxaana lagu cabbiri karaa ama lagu baari karaa iyadoo la adeegsanayo DTNB.

Falanqaynta Xasilloonida ee Isku-dhafka Birta-Sulfur: DTNB waxaa loo isticmaalaa in lagu qiimeeyo xasilloonida iyo fal-celinta isku-dhafka birta-sulfur iyo metalloproteins. Isku-darka wuxuu ogaan karaa oo cabbiri karaa joogitaanka thiols ee ku lug leh chelation-ka ion-yada birta, isagoo bixinaya macluumaad muhiim ah oo ku saabsan isdhexgalka birta-sulfur iyo kiimikada isku-dubaridka.

Baakaynta Alaabta:

Macluumaad Dheeraad ah:

Halabuurka	C14H8N2O8S2
Baaritaan	99%
Muuqaalka	Adkaanta jaallaha ah
Lambarka CAS.	69-78-3
Baakad	Yar iyo weyn
Muddada Khafiska	2 sano
Kaydinta	Ku kaydi meel qabow oo qalalan
Shahaadada	ISO.