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TAPSO CAS:68399-81-5 Qiimaha Soo-saaraha
TAPSO (3-[N-tris(hydroxymethyl)methyl]amino]-2-hydroxypropanesulfonic acid) waa buffer zwitterionic ah oo si caadi ah loogu isticmaalo cilmi-baarista bayoolojiga iyo bayoolojiga. Waa wakiil wax ku ool ah oo buffering ah oo leh pKa oo u dhow pH-ga fiisiyoolajiga, taasoo ka dhigaysa mid ku habboon ilaalinta pH deggan tijaabooyinka bayoolojiga. TAPSO waxaa badanaa loo isticmaalaa nadiifinta borotiinka, tijaabooyinka ensaymka, dhaqanka unugyada, iyo codsiyada electrophoresis. Milaxdeeda biyaha oo sareysa iyo faragelin yar oo ku saabsan hababka bayoolojiga ayaa ka dhigaya doorasho caan ah bulshada sayniska. TAPSO waxaa loo yaqaanaa saameynteeda ugu yar ee dhaqdhaqaaqa ensaymka waxaana badanaa loo isticmaalaa beddel ahaan wakiilada kale ee buffering sida Tris ama fosfate buffers.
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6-Methyl-2-pyridinyl2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside CAS:149263-94-5
6-Methyl-2-pyridinyl 2-(acetylamino)-2-deoxy-1-thio-beta-D-glucopyranoside, oo sidoo kale loo yaqaan N-acetylglucosamine thiolactone, waa isku-dhis kiimiko ah oo leh qaab-dhismeed gaar ah oo ka kooban giraanta pyridine, koox acetylamino ah, iyo qayb thiolactone ah.
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Sodium Glycocholate CAS:863-57-0
Sodium glycocholate waa isku-darka milixda xabaalaha kaas oo ka caawiya dheefshiidka iyo nuugista dufanka cuntada. Waxaa laga sameeyaa glycine iyo cholic acid waxayna door muhiim ah ka ciyaartaa kala-goynta dufanka dhibcaha yaryar, iyadoo kordhinaysa dusha sare si loo dheefshiido si hufan. Sodium glycocholate aad ayuu ugu milmaa biyaha waxaana loo isticmaalaa qaacidooyinka dawooyinka si loo xoojiyo milmida iyo helitaanka bioavailability ee daawooyinka aan si fiican u milmi karin. Intaa waxaa dheer, waxaa loo isticmaalaa cilmi-baarista iyo goobaha shaybaarka si loo taageero koritaanka bakteeriyada iyo noocyada unugyada qaarkood..
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QABOOYINKA cusbada soodhiyamka CAS:70331-82-7
Cusbada sodium-ka ee TAPS, oo sidoo kale loo yaqaan milixda sodium-ka ee N-(Tris(hydroxymethyl)methyl)-2-aminoethanesulfonic acid, waa wakiil wax-ka-qabasho oo kala duwan iyo nidaamiyaha pH-ga. Waxaa badanaa loo isticmaalaa cilmi-baarista bayoolojiga iyo kiimikada, daawooyinka, iyo ogaanshaha. TAPS-Na waa isku-darka zwitterionic kaas oo ka caawiya ilaalinta pH xasilloon oo ku jirta xalalka, taasoo ka dhigaysa mid ku habboon xaalado tijaabo oo kala duwan. Adeegsigeeda waxaa ka mid ah dhaqanka unugyada, cilmi-baarista borotiinka, electrophoresis, isku-darka kiimikada, iyo qaacidooyinka dawooyinka. TAPS-Na waa qaab milmi kara oo deggan oo ka mid ah TAPS kaas oo si fudud loo maareeyo loona isticmaalo xalalka biyaha.
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Koloriin NBD CAS:10199-89-0
NBD chloride, oo sidoo kale loo yaqaan 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, waa rinji fluorescent ah oo si caadi ah loogu isticmaalo codsiyada kiimikada iyo bayoolojiga. Waxay ka tirsan tahay nooca nitrobenzoxadiazole (NBD) derivatives, kuwaas oo muujiya sifooyin fluorescence oo xooggan.
NBD chloride waxaa lagu gartaa awoodda ay u leedahay inay sameysato xiriiro isku xiran oo ay la leedahay nucleophiles, sida amines-ka aasaasiga ah, thiols, iyo kooxaha hydroxyl. Sifadan waxay u oggolaanaysaa in loo isticmaalo sidii wakiil calaamadayn ah oo loogu talagalay biomolecules-ka, oo ay ku jiraan borotiinnada, peptides-ka, asiidhyada nucleic-ga, iyo molecules-ka yaryar.
Marka NBD chloride uu la falgalo nucleophile, wuxuu maraa falcelin beddel ah, taasoo keenta sameynta badeecad calaamadaysan oo NBD ah oo deggan. Badeecadan calaamadaysan waxay muujinaysaa sii deynta iftiinka xooggan, taasoo ka dhigaysa mid waxtar u leh farsamooyinka kala duwan ee ogaanshaha iyo sawir-qaadista.
Qiiqa iftiinka ee molecules-ka calaamadaysan ee NBD waxaa lagu kicin karaa iftiinka ultraviolet (UV) qiyaastii 470 nm, iftiinka la sii daayona waxaa badanaa lagu ogaadaa qiyaastii 530 nm. Mowjadda qiiqan waxay ku dhacdaa gobolka cagaaran ee muuqaalka muuqda, halkaas oo faragelinta ogaanshaha ee muunadaha bayoolojigu ay aad u yar tahay.
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Proteinase K CAS:39450-01-6 Qiimaha Soo-saaraha
Proteinase K waa enzyme serine protease ah oo aad u firfircoon kaas oo kicin kara hydrolysis-ka xidhmooyinka peptide ee borotiinnada. Waxaa laga soo qaatay albamka fungus-ka Engyodontium waxaana loo yaqaanaa noocyo badan oo kala duwan oo gaar ah oo substrate ah. Proteinase K si wax ku ool ah ayuu u kala jari karaa xidhmooyinka peptide dhinaca C-terminal ee asiidhyada hydrophobic, aliphatic, ama aromatic, taasoo ka dhigaysa mid waxtar u leh bayoolajiga molecular-ka iyo codsiyada bayochemistry-ga ee kala duwan..
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N,N-Dimethyl-p-phenylenediamine dihydrochloride CAS:536-46-9
N,N-Dimethyl-p-phenylenediamine dihydrochloride waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo codsiyada sayniska iyo warshadaha kala duwan. Waa budo crystalline ah oo cad ilaa caddaan ah oo leh qaacido molecular ah oo ah C8H14N2·2HCl.
Isku-darkani wuxuu leeyahay isticmaal ballaaran oo kala duwan sababtoo ah sifooyinkiisa iyo fal-celintiisa. Badanaa waxaa loo isticmaalaa sidii falanqayn falanqayn ah oo lagu go'aaminayo peroxides iyo sidoo kale midab dhexdhexaad ah oo lagu soo saaro midabyo iyo midabyo kala duwan.
Dhinaca kiimikada falanqaynta, N,N-Dimethyl-p-phenylenediamine dihydrochloride waxaa badanaa loo isticmaalaa tilmaame midab leh si loo go'aamiyo noocyada oksaydhka, sida hydrogen peroxide ama peroxides ee muunadaha. Isku-darka ayaa isbeddel midab leh ku yimaada falgalka wakiilada oksaydhka, taasoo u oggolaanaysa tiradooda.
Intaa waxaa dheer, isku-darkaan waxaa sidoo kale laga heli karaa qaabaynta rinjiyeynta timaha, halkaas oo uu u dhaqmo sidii horumariye midab. Awooddeeda ay ku abuuri karto midabyo firfircoon oo waara ayaa ka dhigaysa mid caan ka ah warshadaha qurxinta.
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Cusbo disodium ah oo ka samaysan phosphate CAS:922-32-7
Cusbada disodium ee Creatine phosphate waa isku-darka ka kooban creatine, oo ah nooc si dabiici ah u soo saara amino acid, oo ku xiran kooxaha fosfate iyo sodium ions. Waxay door muhiim ah ka ciyaartaa bixinta tamar loogu talagalay murqaha oo isku soo urura waxaana si caadi ah u isticmaala ciyaartoyda iyo jir-dhisayaasha si ay u helaan cunto dheeraad ah.
Marka la cuno, cusbada creatine phosphate disodium waxaa loo beddelaa creatine phosphate oo ku jirta jirka, ka dibna waxaa lagu kaydiyaa muruqyada. Inta lagu jiro hawlaha xooggan, sida qaadista culeyska ama orodka, creatine phosphate waxay u dhaqantaa sidii ilo si fudud loo heli karo oo ah isku-xidhka phosphate-ka ee tamarta sare leh, taasoo u oggolaanaysa dib u buuxinta degdega ah ee adenosine triphosphate (ATP).
ATP waa molecule-ka tamarta ugu muhiimsan ee ay isticmaalaan muruqyadu, heerarkiisuna si degdeg ah ayay hoos ugu dhacaan inta lagu jiro jimicsiga xooggan. Marka lagu daro creatine phosphate, ciyaartoydu waxay kordhin karaan helitaanka ATP, taasoo horseedaysa waxqabadka murqaha oo wanaagsan, awood korodh, iyo daal yar.
Intaa waxaa dheer, cusbada disodium ee creatine phosphate waxay yeelan kartaa faa'iidooyin kale oo suurtagal ah, oo ay ku jiraan kordhinta murqaha, soo kabashada oo la wanaajiyay, iyo shaqada garashada oo la wanaajiyay. Caadiyan waxaa afka laga qaataa qaab budo ama kiniin ah, ha ahaato kabitaan gooni ah ama qayb ka mid ah kabitaan ka hor jimicsiga ama ka dib jimicsiga.
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2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridiniuM-9-carboxylate 4′-NHS Ester CAS:194357-64-7
2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4′-NHS ester waa isku-dhis kiimiko ah oo leh qaab-dhismeed molecular oo adag. Waxay ka kooban tahay koox sulfopylacridinium ah iyo koox shaqeyneysa carboxylate ester. Joogitaanka qaybta ester-ka waxay muujinaysaa inay tahay mid falgal ah oo loo isticmaali karo calaamadayn ama wax ka beddeleysa biomolecules-ka.
Kooxda sulfoplacridinium ee isku-dhafka ah waxay soo jeedinaysaa inay yeelan karto codsiyo suurtagal ah oo ku saabsan tijaabooyinka ku salaysan fluorescence-ka, halkaas oo loo isticmaali karo sidii baaritaan fluorescent ah ama midabayn si loo ogaado loona falanqeeyo biomolecules-ka. Waxa kale oo laga yaabaa inay khusayso daraasadaha hababka unugyada, sida calaamadaynta kaalshiyamka gudaha unugyada.
Ku darista kooxda NHS ester waxay muujinaysaa inay la falgeli karto amines-ka aasaasiga ah, sida kuwa laga helo borotiinnada ama peptides-ka, si ay u sameeyaan xiriirro amide oo deggan. Falcelintani waxay ka dhigaysaa mid waxtar u leh codsiyada bioconjugation, halkaas oo loo isticmaali karo in lagu calaamadeeyo ama wax looga beddelo biomolecules-ka iyadoo la adeegsanayo molecules kale oo shaqeynaya, sida fluorophores ama calaamadaha.
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ABEI CAS: 66612-29-1 Qiimaha soo saaraha
N-(4-Aminobutyl)-N-ethylisoluminol, oo sidoo kale loo yaqaan ABEI, waa isku-dhis iftiin leh oo inta badan loo isticmaalo baaritaanka kiimikada ee codsiyada bayoolojiga. Waxay ka tirsan tahay fasalka isoluminol derivatives waxaana si weyn loogu isticmaalaa baaritaannada difaaca jirka iyo farsamooyinka ogaanshaha DNA/RNA.
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Z-GLY-PRO-PNA CAS:65022-15-3
Z-GLY-PRO-PNA waa peptide nucleic acid (PNA) oo leh taxanaha Z-Gly-Pro. Isku-darkaan waxaa badanaa loo isticmaalaa cilmi-baarista iyo ogaanshaha si loo awoodo inuu si gaar ah ugu xidho taxanaha aashitada niyuukleerka ee dhammaystiran. Ku darista kooxda Z ee N-terminus waxay u oggolaanaysaa bartilmaameed hufan iyo gaarsiinta bartilmaameedyada molecular-ka ee la rabo. Iyada oo leh isku-darka gaarka ah ee sifooyinka peptide iyo nucleic acid, Z-GLY-PRO-PNA waxay bixisaa awood weyn oo ku saabsan codsiyada kala duwan, oo ay ku jiraan daaweynta hidda-wadaha, ogaanshaha molecular-ka, iyo horumarinta daawada..
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TRIS-Acetate CAS:6850-28-8 Qiimaha Soo-saaraha
TRIS-Acetate, waa kayd si caadi ah loogu isticmaalo tijaabooyinka bayoolojiga iyo bayoolojiga. Waa isku-darka saldhigga Tris iyo acetic acid, taasoo keenta xal pH-xasilloon oo loo isticmaalo in lagu xakameeyo oo lagu ilaaliyo heerka pH ee la rabo ee loogu talagalay codsiyo kala duwan. TRIS-Acetate waxay si gaar ah waxtar ugu leedahay daraasadaha DNA iyo RNA, maadaama ay bixiso jawi ku habboon hawlaha ensaymka, electrophoresis, iyo jel electrophoresis. Waxay ka caawisaa ilaalinta xasilloonida iyo hufnaanta asiidhyada nucleic inta lagu jiro hababka kala duwan, sida taxanaha DNA, falgalka silsiladda polymerase (PCR), iyo agarose gel electrophoresis. Marka lagu daro cilmi-baarista asiidhka nucleic, TRIS-Acetate waxaa sidoo kale loo isticmaalaa hababka kala soocidda iyo nadiifinta borotiinka, soo saarista borotiinka xuubka, iyo tijaabooyinka dhaqanka unugyada. Awooddeeda kaydinta ee kala duwan waxay ka dhigaysaa qalab qiimo leh meelo badan oo cilmi-baaris cilmiyeed ah, iyadoo hubinaysa xaalado ugu wanaagsan falcelinta bayoolojiga iyo ilaalinta xasilloonida ensaymada iyo borotiinnada.
