Disodium 4-nitrophenylphosphate CAS:4264-83-9

Saamaynta ugu weyn iyo adeegsiga disodium 4-nitrophenylphosphate waa isticmaalka ay u tahay substrate koromojik ah oo loogu talagalay tijaabooyinka ensaymka alkaline phosphatase. Marka isku-darkaan lagu daro phosphatase alkaline, ensaymku wuxuu kala gooyaa kooxda fosfate-ka disodium 4-nitrophenylphosphate, taasoo keenta sameynta 4-nitrophenol.

4-nitrophenol-ka la soo saaray waa isku-dhis midab jaalle ah oo lagu ogaan karo spectrophotometric ahaan 405 nm. Isbeddelkan midabka waxaa loo isticmaalaa in si tiro ahaan loo cabbiro dhaqdhaqaaqa phosphatase alkaline ee muunadaha, sida dareerayaasha bayoolojiga ama lysates-ka unugyada.

Adeegsiga disodium 4-nitrophenylphosphate ee tijaabooyinka ensaymka wuxuu bixiyaa hab ku habboon oo lagu cabbiro dhaqdhaqaaqa fosfatase alkaline. Ensaymkan wuxuu ku lug leeyahay habab kala duwan oo bayooloji ah waxaana badanaa loo isticmaalaa calaamad muujinaysa kala duwanaanshaha unugyada, shaqada beerka, iyo dheef-shiid kiimikaadka lafaha.

Intaa waxaa dheer, disodium 4-nitrophenylphosphate waxaa loo isticmaali karaa substrate ahaan phosphatases kale, sida acid phosphatase ama protein phosphatases, iyadoo ku xiran dejinta tijaabada iyo yoolka cilmi-baarista.