NBD chloride CAS:10199-89-0 Sawirka La Soo Bandhigay

Koloriin NBD CAS:10199-89-0

NBD chloride, oo sidoo kale loo yaqaan 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, waa rinji fluorescent ah oo si caadi ah loogu isticmaalo codsiyada kiimikada iyo bayoolojiga. Waxay ka tirsan tahay nooca nitrobenzoxadiazole (NBD) derivatives, kuwaas oo muujiya sifooyin fluorescence oo xooggan.

NBD chloride waxaa lagu gartaa awoodda ay u leedahay inay sameysato xiriiro isku xiran oo ay la leedahay nucleophiles, sida amines-ka aasaasiga ah, thiols, iyo kooxaha hydroxyl. Sifadan waxay u oggolaanaysaa in loo isticmaalo sidii wakiil calaamadayn ah oo loogu talagalay biomolecules-ka, oo ay ku jiraan borotiinnada, peptides-ka, asiidhyada nucleic-ga, iyo molecules-ka yaryar.

Marka NBD chloride uu la falgalo nucleophile, wuxuu maraa falcelin beddel ah, taasoo keenta sameynta badeecad calaamadaysan oo NBD ah oo deggan. Badeecadan calaamadaysan waxay muujinaysaa sii deynta iftiinka xooggan, taasoo ka dhigaysa mid waxtar u leh farsamooyinka kala duwan ee ogaanshaha iyo sawir-qaadista.

Qiiqa iftiinka ee molecules-ka calaamadaysan ee NBD waxaa lagu kicin karaa iftiinka ultraviolet (UV) qiyaastii 470 nm, iftiinka la sii daayona waxaa badanaa lagu ogaadaa qiyaastii 530 nm. Mowjadda qiiqan waxay ku dhacdaa gobolka cagaaran ee muuqaalka muuqda, halkaas oo faragelinta ogaanshaha ee muunadaha bayoolojigu ay aad u yar tahay.

Dalbo ama Codso Qiimayn

Faahfaahinta Badeecada

Calaamadaha Alaabta

Codsiga iyo Saamaynta

Calaamadaynta Covalent: NBD chloride waxay samayn kartaa isku xidhnaan covalent ah oo ay la leeyihiin kooxaha nucleophilic ee biomolecules-ka, sida amines-ka aasaasiga ah, thiols, iyo kooxaha hydroxyl. Tani waxay u oggolaanaysaa calaamadayn gaar ah oo loogu talagalay biomolecules-kan si loo ogaado loona sawiro.

Kordhinta Iftiiminta: Marka NBD chloride ay la falgasho nucleophiles, waxay samaysaa badeeco calaamadaysan oo NBD ah oo fluorescent ah. Badeecadan calaamadaysan waxay muujinaysaa sii deynta fluorescent oo xooggan, taasoo ka dhigaysa mid waxtar u leh muuqaalaynta iyo la socodka molecules-ka calaamadaysan.

Xasaasiyadda iyo gaarnimada: Sii deynta iftiinka ee alaabada calaamadaysan ee NBD waa mid aad u xasaasi ah, taasoo u oggolaanaysa ogaanshaha xaddi yar oo ah molecules calaamadaysan. Intaa waxaa dheer, sameynta isku xidhka covalent ee u dhexeeya NBD chloride iyo nucleophiles waxay bixisaa gaar ah, maadaama ay hubinayso ku xidhnaan deggan oo gaar ah oo rinjiga ah oo ku xiran biomolecule-ka bartilmaameedka ah.

Calaamadaynta molecule-ka noolaha: NBD chloride waxaa loo isticmaali karaa in lagu calaamadeeyo molecule-yada noolaha kala duwan, oo ay ku jiraan borotiinno, peptides, asiidhyada nucleic-ga, iyo molecules-yada yaryar. Tani waxay u suurtagelinaysaa cilmi-baarayaasha inay baaraan deegaanka, dhaqdhaqaaqa, iyo isdhexgalka molecule-yadan noolaha ee nidaamyada bayoolojiga.

Mikroskoobka Iftiiminta: Bayoolajiga calaamadaysan ee NBD waxaa lagu arki karaa iyadoo la adeegsanayo mikroskoobka fluorescence. Farsamadani waxay u oggolaanaysaa in la daawado molecules-ka calaamadaysan ee heerka unugyada ama unugyada hoose, iyadoo bixinaysa macluumaad qiimo leh oo ku saabsan qaybintooda iyo dhaqankooda.

Cytometry-ga Socodka: Biomolecules-ka calaamadaysan ee NBD ayaa la falanqeyn karaa oo la qiyaasi karaa iyadoo la adeegsanayo cytometry-ga socodka. Farsamadani waxay u oggolaanaysaa falanqaynta wax-soo-saarka sare ee unugyada calaamadaysan ama walxaha, iyadoo bixinaysa macluumaad ku saabsan heerarka muujinta ama sifooyinka dusha sare ee biomolecules-ka calaamadaysan.

Calaamadaynta iyo raadraaca borotiinka: NBD chloride waxaa loo isticmaali karaa in lagu calaamadeeyo haraaga amino acid gaar ah ama kooxaha shaqeynaya ee borotiinada. Tani waxay u suurtagelinaysaa cilmi-baarayaasha inay bartaan qaab-dhismeedka borotiinka, laablaabashada, dhaqdhaqaaqa, iyo isdhexgalka waqtiga dhabta ah.

Baakaynta Alaabta:

Macluumaad Dheeraad ah:

Halabuurka	C6H2ClN3O3
Baaritaan	99%
Muuqaalka	Budada jaalaha ah
Lambarka CAS.	10199-89-0
Baakad	Yar iyo weyn
Muddada Khafiska	2 sano
Kaydinta	Ku kaydi meel qabow oo qalalan
Shahaadada	ISO.