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Tapso Sodium CAS:105140-25-8 Qiimaha Soo-saaraha
3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic asiidh,Waa anigawakiilka buffering zwitterionic oo ah qaab milix sodium ah. Waxaa si caadi ah loogu isticmaalaa tijaabooyinka bayoolaji iyo bayoolajiga molecular si loo ilaaliyo pH deggan oo ku jira xalalka.It Waa mid aad ugu milma biyaha wuxuuna leeyahay pKa oo u dhow pH-ga jirka, taasoo ka dhigaysa mid ku habboon codsiyo badan sida nadiifinta borotiinka, baaritaanka ensaymka, dhaqanka unugyada, iyo electrophoresis. Qaab-dhismeedkeeda kiimikada ee gaarka ah iyo iswaafajinta nidaamyada bayoolojiga ayaa ka dhigaya qayb muhiim ah oo ku jirta hab-raacyada shaybaarka ee kala duwan.
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4-Nitrophenyl phosphate di(tris) cusbo CAS:68189-42-4
Cusbada 4-Nitrophenyl phosphate di(tris) waa isku-dhis kiimiko ah oo loo isticmaalo cilmi-baarista iyo ogaanshaha kiimikada. Waxaa badanaa loo isticmaalaa sidii substrate loogu talagalay tijaabooyinka enzyme-ka alkaline phosphatase. Cusbadaan, marka lagu shubo phosphatase alkaline, waxay soo saartaa badeecad midab jaalle ah oo si fudud loogu cabbiri karo spectrophotometric. Falcelintan waxaa si weyn loogu isticmaalaa codsiyo kala duwan, oo ay ku jiraan baaritaannada immunosorbent-ka ee enzyme-ku xiran (ELISAs) iyo ogaanshaha dhaqdhaqaaqa phosphatase. Xoogga midabka jaallaha ah wuxuu si toos ah ugu dhigmaa dhaqdhaqaaqa enzyme-ka, taasoo u oggolaanaysa qiyaas sax ah oo heerarka phosphatase alkaline ee muunadaha bayoolojiga. Cusbada 4-Nitrophenyl phosphate di(tris) waa mid deggan, biyo-milmi karta, aadna u xasaasi ah, taasoo ka dhigaysa doorasho caan ah oo loogu talagalay baaritaannada enzyme-ka ee cilmi-baarista bayoolojiga iyo caafimaadka.
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Nitrotetrazolium chloride buluug ah CAS: 298-83-9
Nitrotetrazolium blue chloride, oo sidoo kale loo yaqaan NBT ama NBT-Cl, waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo baaritaannada bayoolojiga iyo bayoolojiga. Waa budo crystalline ah oo huruud khafiif ah leh oo ku milma biyaha.
NBT waxaa badanaa loo isticmaalaa tilmaame si loo ogaado jiritaanka xagjirrada superoxide ama enzymes-ka soo saara. Waxay martaa isbeddel midab marka ay yareeyaan xagjirrada superoxide, iyadoo ka beddeleysa huruud una beddelaysa soo-baxa formazan buluug qoto dheer. Falcelintan waxaa badanaa loo adeegsadaa tijaabooyinka lagu barto walbahaarka oksaydhka, neefsashada unugyada, iyo dhaqdhaqaaqa ensaymka.
NBT waxaa badanaa loo adeegsadaa farsamooyin kala duwan, sida dhimista nitroblue tetrazolium, si loo arko wax soo saarka superoxide ee unugyada iyo unugyada. Waxa kale oo loo isticmaalay immunohistochemistry iyo histochemistry si loo ogaado loona helo ensaymyada kale iyo qaybaha unugyada.
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NSP-AS CAS:211106-69-3 Qiimaha Soo-saaraha
Cusbada gudaha ee isku-dhafka ah ee 3-[9-(((3-(carboxypropyl)[4-Methxylphenyl]sulfonyl)aMine)carboxyl]-10-acridiniuMyl)-1-propanesulfonate waa molecule isku dhafan oo ka kooban qayb acridinium ah oo ku xiran cusbo gudaha ah oo propanesulfonate ah. Waxay ka kooban tahay koox carboxypropyl ah, koox 4-Methxylphenylsulfonyl ah, iyo koox amine ah. Isku-dhafkan waxaa loo isticmaali karaa si gaar ah meelaha sida kiimikada, daawooyinka, ama cilmi-baarista.
Intaa waxaa dheer, NAC waxay muujisay ballanqaad ah inay taageerto caafimaadka beerka iyadoo gacan ka geysaneysa ka saarista walxaha sunta ah, sida acetaminophen, oo ah daawo xanuun baabi'isa oo caadi ah. Waxa kale oo laga yaabaa inay leedahay saameyn difaac ah oo ka dhan ah dhaawaca beerka ee uu keeno isticmaalka khamriga.
Marka laga soo tago sifooyinka antioxidant-ka iyo taageerada neefsashada, NAC waxaa lagu sameeyay sahaminta faa'iidooyinka suurtagalka ah ee caafimaadka dhimirka. Cilmi-baaris qaar ayaa soo jeedinaysa inay saameyn togan ku yeelan karto khalkhalka niyadda, sida niyad-jabka iyo khalkhalka qasabka ah ee obsessive-compulsive (OCD).
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L-Leucine p-nitroanilide hydrochloride CAS: 16010-98-3
L-Leucine p-nitroanilide hydrochloride waa isku-dhis kiimiko ah oo ka tirsan kooxda walxaha p-nitroanilide laga sameeyay. Waa budo crystalline ah oo cad ilaa caddaan ah oo si caadi ah loogu isticmaalo cilmi-baarista bayoolaji iyo tijaabooyinka ensaymka.
L-Leucine p-nitroanilide hydrochloride waxaa badanaa loo isticmaalaa substrate si loo cabbiro dhaqdhaqaaqa enzymes-ka sida proteases iyo peptidases. Marka enzymes-kani ay kala gooyaan L-Leucine p-nitroanilide hydrochloride, waxay sii deysaa p-nitroaniline, kaas oo lagu ogaan karo spectrophotometry ahaan. Heerka isbeddelka midabka ayaa si toos ah ugu dhigma dhaqdhaqaaqa enzymatic-ka, taasoo u oggolaanaysa qiyaasta dhaqdhaqaaqa enzymatic-ka ee muunadaha bayoolojiga.
Isku-darkani wuxuu ku milmaa biyaha waxaana si fudud loogu milmi karaa xalal ku habboon oo loogu talagalay in lagu isticmaalo tijaabooyinka enzymatic. Waa muhiim in lagu kaydiyo L-Leucine p-nitroanilide hydrochloride meel qabow oo qalalan si loo ilaaliyo xasilloonidiisa.
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Guanidine Thiocyanate Sulfate CAS:593-84-0
Guanidine thiocyanate sulfate waa isku-dhis kiimiko ah oo laga soo qaatay guanidine, thiocyanate, iyo sulfate ions. Waxaa si caadi ah loogu isticmaalaa bayoolajiga molecular-ka iyo cilmi-baarista bayochemistry-ga iyadoo ah wakiil chaotropic ah.
Guanidine thiocyanate sulfate waxaa badanaa loo isticmaalaa hab-raacyada kala-goynta DNA iyo RNA si loo carqaladeeyo xuubka unugyada iyo borotiinnada denature, taasoo u oggolaanaysa soo saarista hufan ee asiidhyada nucleic-ga. Waxay ka caawisaa milmida DNA-da iyo RNA, taasoo suurtogalinaysa in la kala saaro oo laga nadiifiyo muunado kala duwan oo bayooloji ah.
Iyada oo ay ugu wacan tahay sifooyinka chaotropic-ga, guanidine thiocyanate sulfate wuxuu ka caawiyaa jebinta isku xidhka haydarojiin iyo isdhexgalka kale ee aan isku xidhnayn, isagoo fududeynaya kala soocida asiidhyada nucleic-ga iyo borotiinnada, dufanka, iyo qaybaha kale ee unugyada. Waxay sidoo kale ka caawisaa joojinta dhaqdhaqaaqa RNase, iyadoo hubinaysa hufnaanta iyo daahirnimada asiidhyada nucleic-ga ee la soo saaray..
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BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine cusbo CAS:6578-06-9
BCIP-Toluidine, oo sidoo kale loo yaqaan cusbada 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo cilmi-baarista bayoolajiga molecular-ka iyo bayochemistry-ga. Waxaa ugu horreyn loo isticmaalaa substrate-ka baaritaanka immunosorbent-ka ee enzyme-linked (ELISAs) ama farsamooyinka rinjiyeynta immunohistochemical si loo ogaado dhaqdhaqaaqa phosphatase alkaline.
Marka BCIP-Toluidine lagu kala jaro fosfatase alkaline, waxay martaa falgal isbeddel midab ah, taasoo soo saarta daadasho buluug-guduud ah. Falcelintani waxay u oggolaanaysaa muuqaalka iyo cabbirka dhaqdhaqaaqa fosfatase alkaline ee muunadaha. Xoogga midabka waxaa lagu cabbiri karaa si sawir-qaade ah waxayna si toos ah ugu dhigantaa dhaqdhaqaaqa ensaymka.
Isticmaalka BCIP-Toluidine oo ah substrate gaar ahaan waa wax caadi ah marka la adeegsanayo calaamadaynta DNA ama RNA, sida in situ hybridization (ISH) ama Southern and Northern blotting. Waxay bixisaa hab lagu kalsoonaan karo oo xasaasi ah oo lagu ogaanayo jiritaanka asiidhyada nucleic-ga ee bartilmaameedka ah ee muunadaha.
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3-Aminophthalhydrazide CAS:521-31-3
3-Aminophthalhydrazide waa isku-dhis kiimiko ah oo leh qaacidada molecular-ka C8H8N2O. Waxaa loogu yeeraa aminohydrazide oo ka soo jeeda phthalic acid. Isku-darkani wuxuu ka kooban yahay laf-dhabar phthalic acid ah oo leh laba atam oo haydarojiin ah oo lagu beddelay koox amino ah iyo koox hydrazine ah.
3-Aminophthalhydrazide waxay leedahay adeegsiyo kala duwan oo ku saabsan kiimikada dabiiciga ah iyo daawada. Waxaa loo isticmaali karaa sidii agab bilow ah oo lagu sameeyo isku-darka daawooyinka kala duwan iyo midabaynta. Intaa waxaa dheer, waxaa badanaa loo isticmaalaa diyaarinta saldhigyada Schiff, kuwaas oo ah dhexdhexaad muhiim ah oo ku jira isku-darka dabiiciga ah.
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4-Aminoantipyrine CAS:83-07-8
4-Aminoantipyrine, oo sidoo kale loo yaqaan aminoantipyrine ama aminopyrine, waa isku-dhis dabiici ah oo macmal ah. Waxaa badanaa loo isticmaalaa sidii reagent-ka kiimikada falanqaynta iyo warshadaha dawooyinka. Qaab-dhismeedkeeda kiimikada wuxuu ka kooban yahay giraan udgoon oo leh koox amino (NH2) iyo koox pyrazolone ah, taasoo ka dhigaysa isku-dhis badan oo loogu talagalay codsiyada kala duwan.
Kiimikada falanqaynta, 4-Aminoantipyrine waxaa si weyn loogu isticmaalaa sidii falgal koromosoom ah oo ku jira tijaabooyinka midabka. Waxay la falgashaa walxo gaar ah, sida phenols iyo peroxides, si ay u sameeyaan isku-dhafan midab leh oo lagu cabbiri karo si sawir-qaade ah. Sifadan waxay ka dhigaysaa mid waxtar leh baaritaanno kala duwan, oo ay ku jiraan go'aaminta ensaymada sida peroxidase, glucose, iyo amylase.
Warshadaha dawooyinka, 4-Aminoantipyrine waxaa taariikh ahaan loo isticmaali jiray daawo xanuun baabi'iye iyo qandho jabis ah. .
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TAPS-NA CAS:91000-53-2 Qiimaha Soo-saaraha
Cusbada sodium ee N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid, oo sidoo kale loo yaqaan TAPS-Na, waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo sidii wakiil kaydin ah oo loogu talagalay codsiyada bayoolojiga iyo bayoolaji ee kala duwan. Waa isku-dhis zwitterionic ah, taasoo la macno ah inay leedahay labadaba dallacaad togan iyo taban, taasoo ka caawisa ilaalinta pH deggan oo ku jirta xalalka.
TAPS-Na waxay leedahay kala duwanaansho pH ah oo u dhaxaysa 7.7 ilaa 9.1, taasoo ka dhigaysa mid ku habboon codsiyada u baahan jawi pH ah oo alkaline ah. Badanaa waxaa loo isticmaalaa cilmi-baarista borotiinka iyo ensaymka, iyo sidoo kale farsamooyinka bayoolajiga molecular-ka sida electrophoresis-ka jel iyo taxanaha DNA-da.
Sida cusbo soodhiyam ah, TAPS-Na aad ayuu ugu milmaa biyaha, taasoo u oggolaanaysa diyaarinta fudud ee xalalka la keydiyay. Waxaa loo yaqaanaa sunta yar iyo faragelinta yar ee molecules-ka bayoolojiga, taasoo ka dhigaysa doorasho la doorbido tijaabooyin badan.
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5,5-Dithiobis(2-nitrobenzoic acid) CAS:69-78-3
5,5-Dithiobis (2-nitrobenzoic acid), oo sidoo kale loo yaqaan DTNB ama reagent Ellman, waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo cilmi-baarista kiimikada iyo dawooyinka. Waa budo huruud ah oo crystalline ah oo si aad ah ugu milma biyaha iyo dareerayaasha dabiiciga ah.
DTNB waxaa ugu horreyn loo isticmaalaa in lagu cabbiro isku-darka kooxaha sulfhydryl (-SH) ee borotiinnada iyo peptides-ka. Isku-darka wuxuu la falgalaa thiols, taasoo keenta sameynta disulfide isku dhafan oo sii daaya 2-nitro-5-thiobenzoate, kaas oo leh midab jaalle ah oo lagu cabbiri karo spectrophotometric. Marka la cabbiro xoogga midabka jaallaha ah, waxaa suurtagal ah in la qiyaaso isku-darka thiols ee muunad. Farsamadan waxaa badanaa loo isticmaalaa in lagu barto isdhexgalka borotiinka-borotiinka, laabashada borotiinka, iyo falcelinta ensaymikada ee ku lug leh kooxaha thiol.
Marka laga soo tago qiyaasta thiol, DTNB waxaa loo adeegsaday codsiyada kale, sida qiimeynta xaaladda redox ee unugyada, barashada xasilloonida isku-dhafka birta-sulfur, iyo cabbiraadda dhaqdhaqaaqa enzymes-ka kala duwan.
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PHENOLPHTHALEIN MONOPHOSPHATE BIS(CYCLOHEXYLAMMONIUM) CUSBO CAS:14815-59-9
Cusbada Phenolphthalein monophosphate dicyclohexylammonium waa isku-dhis kiimiko ah oo laga soo qaatay phenolphthalein, oo ah tilmaame pH ah oo si caadi ah loogu isticmaalo goobaha shaybaarka. Qaabka cusbada dicyclohexylammonium ee phenolphthalein monophosphate waxaa badanaa loo isticmaalaa sidii falanqayn falanqayn ah oo ku saabsan cilmi-baarista bayoolaji iyo molecular bayooloji. Waxaa ugu horreyn loo isticmaalaa tijaabooyinka ama tijaabooyinka si loo ogaado oo loo cabbiro jiritaanka ensaymyo gaar ah, oo ay ku jiraan kuwa ku lug leh hawlaha ATP (adenosine triphosphate) hydrolysis ama phosphatase. Sifooyinkeeda gaarka ah waxay ka dhigayaan qalab waxtar leh baaritaannada sayniska ee la xiriira kinetics ensaym iyo hababka unugyada..
