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2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2
2-Nitrophenyl-beta-D-glucopyranoside waa kiimiko ka kooban molecule glucopyranoside oo ku xiran koox nitrofenyl ah.Waxaa caadi ahaan loo isticmaalaa sida substrate ee imtixaannada enzymatic si loo ogaado oo loo qiyaaso dhaqdhaqaaqa enzymes sida beta-glucosidase.Kooxda nitrophenyl waxaa lagu kala jeexjeexi karaa enzyme-ka, taasoo keentay in la sii daayo badeeco midab jaale ah oo lagu qiyaasi karo spectrophotometrically.Xaruntani waxay si gaar ah faa'iido u leedahay barashada kinetics-ka enzymka iyo baadhista sare u kaca ee ka hortagga enzyme-ka ama firfircoonayaasha.Waxa kale oo ay ka shaqeysaa cilmi-baarista biochemical ee baaritaanka dheef-shiid kiimikaadka karbohaydraytyada iyo sidii substrate-gaar ah-glycosidic-linkage.
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Tris Base CAS: 77-86-1 Qiimaha soo saaraha
Tris Base, oo sidoo kale loo yaqaan Tromethamine ama THAM, waa iskudhis dabiici ah oo caadi ahaan loo isticmaalo goobta biochemistry iyo bayoolaji molecular.Waa cad, budo crystalline ah oo aad ugu milmay biyaha oo leh ur amine ah oo lagu garto.Tris Base waxaa inta badan loo isticmaalaa sidii wakiilka kaydinta si loo ilaaliyo pH deggan tijaabooyinka iyo hababka bayoolojiga ee kala duwan, sida DNA iyo daraasaadka borotiinka.Waxa kale oo loo isticmaali karaa samaynta dawooyinka iyo soo saarista wakiilada firfircoon ee dusha sare leh.Guud ahaan, Tris Base waa qayb muhiim ah oo ka mid ah codsiyada shaybaarada badan halkaas oo ilaalinta pH saxda ah ay muhiim tahay.
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4-Morpholineethanesulfonic acid CAS:4432-31-9
4-Morpholineethanesulfonic acid, oo caadi ahaan loo yaqaan MES, waa iskudhis zwitterionic ah oo u adeegta sidii wakiilka kaydinta cilmi-baarista bayooloji iyo kiimikaad.Waxay ka caawisaa in la ilaaliyo pH deggan agagaarka 6-7.5 waxaana si ballaaran loo isticmaalaa sababtoo ah sunta hoose ee hoose iyo waafaqsanaanta hababka noolaha iyo enzymes kala duwan.MES waxaa loo isticmaalaa electrophoresis, daraasaadka enzyme, dhaqanka unugyada, nadiifinta borotiinka, iyo hababka kale ee tijaabada ah ee u baahan xakamaynta pH ee saxda ah.
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4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7
4-Nitrophenyl-beta-D-glucopyranoside waa substrate caadi ahaan loo isticmaalo tijaabooyinka kiimikada si loo qiimeeyo dhaqdhaqaaqa enzymes sida β-glucuronidase.Xaruntan waxaa hydrolyzed by enzyme, taasoo keentay in la sii daayo 4-nitrophenol, kaas oo lagu qiyaasi karaa iyadoo la isticmaalayo spectrophotometry.Adeegsigeedu waxa uu u sahlayaa cilmi-baarayaasha in ay daraasad ku sameeyaan dhinacyo kala duwan oo ku saabsan dheef-shiid kiimikaad ee daroogada, toxicology, iyo ogaanshaha caafimaad ee la xidhiidha falcelinta glucuronidation.
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MOPSO sodium cusbo CAS: 79803-73-9
MOPSO sodium cusbo waa iskudhis kiimiko ah oo laga soo qaatay MOPS (3-(N-morpholino)propanesulfonic acid).Waa milix buffer zwitterionic ah, taasoo la micno ah inay ka kooban tahay labadaba lacag togan iyo mid taban, taas oo u oggolaanaysa inay si wax ku ool ah u ilaaliso xasilloonida pH ee tijaabooyinka bayoolojiga iyo biochemical ee kala duwan.
Nooca cusbada sodium ee MOPSO waxay bixisaa faa'iidooyin ay ka mid yihiin hagaajinta xalalka aqueous, taasoo sahlaysa in la xakameeyo oo la diyaariyo.Waxaa caadi ahaan loo isticmaalaa sidii wakiilka wax-ka-qabashada ee warbaahinta dhaqanka unugga, farsamooyinka bayoolajiga molecular, falanqaynta borotiinka, iyo falcelinta enzyme.
MOPSO sodium cusbada waxay caawisaa ilaalinta pH ee dhexdhexaadka koritaanka ee dhaqanka unugyada, siinta jawi deggan koritaanka unugyada iyo shaqada.Farsamooyinka bayoolajiga molecular, waxay dejinaysaa pH ee isku-dhafka falcelinta iyo socodsiinta, hubinta natiijooyinka saxda ah ee la isku halayn karo ee DNA iyo RNA go'doominta, PCR, iyo electrophoresis jel.
Waxa kale oo loo adeegsadaa falanqaynta borotiinka, iyada oo u shaqaynaysa sidii wakiilka buffering inta lagu jiro nadiifinta borotiinka, qiyaasidda, iyo electrophoresis.MOPSO sodium cusbada waxay hubisaa xaaladaha pH ee ugu fiican ee xasiloonida borotiinka iyo dhaqdhaqaaqa inta lagu jiro hababkan.
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4-Methylumbelliferyl-beta-D-glucopyranoside CAS: 18997-57-4
4-Methylumbelliferyl-beta-D-glucopyranoside waa substrate caadi ahaan loo isticmaalo qiyaasaha enzymatic si loo barto dhaqdhaqaaqa beta-glucosidase enzymes.Marka lagu dhaqmo beta-glucosidase, waxay maraysaa hydrolysis, taasoo keentay in la sii daayo 4-methylumbelliferone, kaas oo lagu ogaan karo oo lagu qiyaaso iyadoo la isticmaalayo fluorescence spectroscopy.Xaruntan waxaa si weyn loogu isticmaalaa dhinacyada biochemistry, bayoolaji molecular, iyo bayotechnoolajiyada baaritaanka dhaqdhaqaaqa enzyme iyo ujeedooyinka baaritaanka.Hantideeda fluorescence waxay ka dhigaysa mid xasaasi ah oo ku habboon codsiyada sare-u-qaadista.
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METHYL Beta-D-GLUCOPYRANOSIDE HEMIHYDRATE CAS:7000-27-3
Methyl beta-D-glucopyranoside hemihydrate waa unug kiimiko ah oo ka tirsan fasalka glucopyranosides.Waa budo crystalline cad oo biyo ku milmay.Xaruntan waxaa sida caadiga ah loo isticmaalaa ilaha karbohaydrayt ee warbaahinta dhaqanka unugga iyo substrate falcelinta enzymatic ee cilmi-baarista bayooloji iyo baayooloji.Waxay u adeegi kartaa qaab dhismeed si loo barto dheef-shiid kiimikaadka karbohaydraytyada, gaadiidka, iyo ka faa'iidaysiga hababka noole ee kala duwan.Methyl beta-D-glucopyranoside hemihydrate waxay ka heshaa codsiyada goobta glycobiology, enzymology, iyo horumarinta daroogada, halkaas oo loo isticmaalo sidii qalab qalab ah oo loogu talagalay tijaabooyin kala duwan iyo tijaabooyin.
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AMPSO CAS: 68399-79-1 Qiimaha soo saaraha
AMPSO, ama 3-[(1,1-dimethyl-2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid, waa kaydka zwitterionic oo inta badan loo isticmaalo cilmi-baarista bayooloji iyo kiimikaad.Waxay leedahay qiimaha pKa ee agagaarka 7.9, taas oo ka dhigaysa mid ku habboon ilaalinta xaaladaha pH ee deggan ee goobaha tijaabada ah ee kala duwan.AMPSO waxaa badanaa lagu isticmaalaa warbaahinta dhaqanka unugyada, nadiifinta borotiinka, baaritaanka enzymes, jeliyeyaasha electrophoresis, iyo DNA-da.Waxay ka caawisaa ilaalinta qiyaasta pH ee la rabo, iyada oo hubinaysa xaaladaha ugu fiican ee koritaanka unugyada, xasiloonida borotiinka, dhaqdhaqaaqa enzyme, iyo kala-soocidda saxda ah iyo falanqaynta biomolecules. Iyada oo awooddeeda u leh inay iska caabiso isbeddellada pH ee ay keento ku darida acids ama saldhigyada, AMPSO waa qalab qiimo leh ilaalinta kontoroolka saxda ah ee pH ee tijaabooyin kala duwan oo bayooloji iyo bayooloji ah.
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Bis-tris hydrochloride CAS: 124763-51-5
Bis-tris hydrochloride waa iskudhis leh sifooyin kaydin oo caadi ahaan loo isticmaalo tijaabooyinka bayoloji iyo kiimikaad.Waxay ka caawisaa in ay joogteyso pH xasiloon waxaana loo adeegsadaa borotiinka electrophoresis, imtixaannada waxqabadka enzyme, dhaqanka unugyada, iyo qaababka dawooyinka.Shaqadeeda ugu weyn waa in ay iska caabiso isbeddelada pH marka asiidhyada ama saldhigyada lagu daro xalka, taas oo ka dhigaysa qalab lagama maarmaan u ah codsiyada sayniska iyo warshadaha kala duwan.
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Alpha-D-Glucose pentaacetate CAS:3891-59-6
Alpha-D-glucose pentaacetate waa kiimiko kiimiko ah oo lagu helo acetylating kooxaha hydroxyl ee alfa-D-glucose oo leh shan kooxood oo acetyl ah.Waxaa caadi ahaan loo isticmaalaa isku-dhafka dabiiciga ah sida koox ilaalin ah kooxaha hydroxyl ee ku jira karbohaydraytyada.Waxa kale oo loo isticmaali karaa tixraac tixraac ah oo ku saabsan cilmi-baarista iyo falanqaynta kiimikada, iyo sida walxaha bilawga ah ee isku-dhafka xeryahooda kala duwan.Intaa waxaa dheer, gulukoos pentaacetate ayaa lagu baaray isticmaalka suurtagalka ah ee hababka gudbinta daroogada, sababtoo ah sifooyinkeeda la xakameynayo.
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AMPD CAS: 115-69-5 Qiimaha soo saaraha
2-Amino-2-methyl-1,3-propanediol, oo sidoo kale loo yaqaan AMPD ama α-methyl serinol, waa unug kiimiko oo leh caanaha molecular C4H11NO2.Waa aalkolo amino ah oo sida caadiga ah loo isticmaalo dhexdhexaadinta kiimikaad ee isku dhafka daawooyinka iyo xeryahooda organic.AMPD waxay caan ku tahay awooda ay u leedahay inay u dhaqanto sidii caawiye chiral falcelinta asymmetric, taasoo ka dhigaysa mid qiimo leh soo saarista xeryahooda saafiga ah.Intaa waxaa dheer, waxa loo isticmaalay sidii qayb ka mid ah daryeelka shakhsi ahaaneed iyo alaabta la isku qurxiyo ee guryaha qoyaanka ah.
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KOOXDA SODIUMKA CUSBADA AH: 105140-23-6
CAPS sodium cusbada waa kaydka zwitterionic sida caadiga ah loo isticmaalo codsiyada bayooloojiyada molecular.Waxay leedahay qiimaha pKa ee ku dhawaad 10.4, taas oo ka dhigaysa mid waxtar u leh pH inta u dhaxaysa 9.7 iyo 11.1.CAPS sodium cusbada waxaa loo isticmaalaa borotiinka electrophoresis, falcelinta enzymatic, bayooloji iyo kiimikaad qiimaynta, iyo warbaahinta dhaqanka unugyada.Waxay bixisaa iska caabin ah isbeddellada pH ee ay keento wasakhaysan waxayna leedahay milmayn wanaagsan oo biyaha ah.
