4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Saamaynta 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) waa inay u adeegto sidii substrate enzyme beta-glucosidase. Ensaymkan wuxuu kala gooyaa isku xirka glucosidic ee MUG, taasoo keenta sii deynta 4-Methylumbelliferone (4-MU). Adeegsiga MUG wuxuu ugu horreyn ku jiraa cilmiga microbiology, gaar ahaan ogaanshaha iyo aqoonsashada bakteeriyada soo saarta beta-glucosidase. MUG waxaa badanaa loo isticmaalaa ogaanshaha Escherichia coli (E. coli) ee biyaha iyo muunadaha cuntada. E. coli wuxuu leeyahay enzyme beta-glucosidase, kaas oo hydrolyze MUG ku samayn kara oo soo saari kara calaamad fluorescent ah iyadoo ay jirto iftiinka ultraviolet (UV). Hantida fluorescent ee 4-MU waxay u oggolaanaysaa si fudud loo ogaado oo loo cabbiro. Marka substrate-ka MUG la hydrolyzed, 4-MU ee la soo saaray wuxuu soo saaraa fluorescence buluug ah, taasoo sahlaysa aqoonsiga bakteeriyada leh dhaqdhaqaaqa beta-glucosidase. Habkani waxaa badanaa loo isticmaalaa kormeerka deegaanka iyo tijaabinta badbaadada cuntada, maadaama uu bixiyo hab degdeg ah oo xasaasi ah oo lagu ogaanayo wasakhowga bakteeriyada. Marka laga soo tago isticmaalkeeda microbiology, MUG waxaa sidoo kale loo isticmaali karaa in lagu barto dhaqdhaqaaqa iyo joojinta beta-glucosidase ee cilmi-baarista bayoolajiga iyo bayoolajiga molecular. Iftiinkeedu wuxuu suurtogal ka dhigayaa cabbirka kinetics-ka ensaymka waxaana loo isticmaali karaa in lagu baaro waxyaabaha ka hortagaya ama firfircoonayaasha dhaqdhaqaaqa beta-glucosidase. Guud ahaan, MUG waa isku-dhafan kala duwan oo hela codsi ballaaran oo ku saabsan beeraha microbiology, biochemistry, iyo bayoolajiga molecular si loo ogaado dhaqdhaqaaqa beta-glucosidase iyo aqoonsiga bakteeriyada soo saarta ensaymkan.