4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7

Tijaabinta dhaqdhaqaaqa enzyme-ka: Hydrolysis-ka 4-Nitrophenyl-beta-D-glucopyranoside ee ay sameeyaan ensaymada sida β-glucosidase ayaa loo isticmaali karaa in lagu cabbiro dhaqdhaqaaqa enzyme-ka. Sii deynta 4-nitrophenol waxaa lagu qiyaasi karaa iyadoo la adeegsanayo spectrophotometry, taasoo u oggolaanaysa go'aaminta kinetics-ka enzyme-ka iyo daraasadaha joojinta.

Tijaabooyinka hiddaha iyo baaritaanka wariyaha: 4-Nitrophenyl-beta-D-glucopyranoside waxaa lagu dari karaa calaamado gaar ah oo molecular ah oo lagu daray borotiinada isku-dhafka. Dhaqdhaqaaqa ensaymka ee borotiinka isku-dhafka ayaa markaa si fudud loo cabbiri karaa iyadoo la cabbirayo sii deynta 4-nitrophenol, taasoo bixinaysa aragtiyo ku saabsan muujinta hidda-wadaha iyo isdhexgalka borotiinka-borotiinka.

Soo saarista daawada iyo baaritaanka wax soo saarka sare: Hirgelinta 4-Nitrophenyl-beta-D-glucopyranoside waxaa loo isticmaali karaa tijaabo waxqabad oo beddel ah oo lagu ogaanayo daawada. Waxay u oggolaanaysaa baaritaanka degdegga ah ee maktabadaha isku-dhafka ah ee waaweyn si loo aqoonsado xannibaadaha ensaymka ama firfircoonayaasha suurtagalka ah.

Baaritaanka ogaanshaha: Cudurrada qaar waxay la xiriiraan isbeddellada ku yimaada hawlaha ensaymyada qaarkood. Iyadoo la adeegsanayo hydrolysis-ka 4-Nitrophenyl-beta-D-glucopyranoside iyadoo la adeegsanayo ensaymyo gaar ah oo loo adeegsado baaritaan ahaan, cudurradan waa la ogaan karaa ama la socon karaa. Tan waxaa ka mid ah xaaladaha la xiriira khalkhalka kaydinta lysosomal iyo cudurrada beerka.