Phenylgalactoside CAS: 2818-58-8

Saamaynta hawsha enzyme: Phenylgalactoside waxaa caadi ahaan loo isticmaalaa in lagu cabbiro hawsha enzyme β-galactosidase.Marka phenylgalactoside lagu hydrolyzed by β-galactosidase, waxa ay sii daayaan p-nitrophenol.Ururinta p-nitrophenol waxaa lagu qiyaasi karaa tiro ahaan, iyadoo la siinayo fikrado ku saabsan waxqabadka β-galactosidase.Saamayntan waxaa lagu qalabeeyaa codsiyada sida baaritaanka enzymes iyo hababka baaritaanka.

Falanqaynta muujinta hidda-wadaha: Phenylgalactoside waxaa inta badan loo adeegsadaa sida substrate ee tijaabooyinka bayoolajiga molecular si loo barto muujinta hidda-wadaha.Hidde-sidaha lacZ, kaas oo qeexaya β-galactosidase, ayaa caadi ahaan lagu dhex daray nidaamyo nidaamsan oo hiddo-wadaha kale ee xiisaha leh.Muujinta hidda-wadaha lacZ iyo hydrolysis of phenylgalactoside by β-galactosidase waxay muujin kartaa qaabka muujinta iyo heerka hiddasidaha bartilmaameedka ah ee la baranayo.

Nidaamyada baarista: Phenylgalactoside waa qayb muhiim ah oo ka mid ah hababka baarista ee ka faa'iideysanaya waxqabadka β-galactosidase.Mid ka mid ah tusaalaha si weyn loo yaqaan ayaa ah habka baarista buluug-caddaan, kaas oo loo isticmaalo in lagu aqoonsado unugyada dib-u-habaynta ama isbeddelka ee tijaabooyinka bayoolaji molecular.Gumeysiga si guul leh u qaatay DNA-da dib-u-habaynta ama dib-u-habaynta hidde-sidaha ayaa muujin doona β-galactosidase, taasoo horseedaysa hydrolysis ee phenylgalactoside iyo samaynta midab buluug ah.

Nadiifinta borotiinka: Xaaladaha qaarkood, phenylgalactoside waxaa loo isticmaali karaa sidii ligand loogu talagalay chromatography affinity si loo nadiifiyo borotiinnada si gaar ah ugu xidha ama loo hawlgeliyo β-galactosidase.Borotiinka xiisaha waxa laga yaabaa inuu lahaado sumad xidhiidhsan ama sumad fiyuus oo ka kooban β-galactosidase-binding domain.Marka la dhex mariyo isku darka borotiinka iyada oo loo marayo tiir leh phenylgalactoside aan dhaqdhaqaaq lahayn, borotiinka la rabo ayaa si xushmad leh loo hayn karaa ka dibna waa la nadiifin karaa.