Phenylgalactoside CAS:2818-58-8

Saamaynta dhaqdhaqaaqa ensaymka: Phenylgalactoside waxaa badanaa loo isticmaalaa in lagu cabbiro dhaqdhaqaaqa ensaymka β-galactosidase. Marka phenylgalactoside lagu shubo β-galactosidase, waxay sii deysaa p-nitrophenol. Uruurinta p-nitrophenol waxaa lagu cabbiri karaa tiro ahaan, taasoo bixinaysa aragtiyo ku saabsan dhaqdhaqaaqa β-galactosidase. Saamayntan waxaa lagu isticmaalaa codsiyada sida baaritaannada ensaymka iyo nidaamyada baaritaanka.

Falanqaynta muujinta hidda-wadaha: Phenylgalactoside waxaa badanaa loo isticmaalaa sidii substrate tijaabooyinka bayoolajiga molecular si loo barto muujinta hidda-wadaha. Hidda-wadaha lacZ, oo codeeya β-galactosidase, ayaa badanaa lagu daraa taxanaha sharciyeynta ee hidda-wadaha kale ee xiisaha leh. Muujinta hidda-wadaha lacZ iyo hydrolysis-ka phenylgalactoside ee β-galactosidase waxay tilmaami kartaa qaabka muujinta iyo heerka hidda-wadaha bartilmaameedka ah ee la baranayo.

Nidaamyada Baaritaanka: Phenylgalactoside waa qayb muhiim ah oo ka mid ah nidaamyada baaritaanka ee isticmaala dhaqdhaqaaqa β-galactosidase. Tusaale ahaan si weyn loo yaqaan waa habka baaritaanka buluugga-cad, kaas oo loo isticmaalo in lagu aqoonsado unugyada dib-u-isku-dhafan ama kuwa isbeddelay ee tijaabooyinka bayoolajiga molecular. Gumeysiyada si guul leh u qaatay DNA-da dib-u-isku-dhafan ama maray isku-dhafka hidde-sidaha ayaa muujin doona β-galactosidase, taasoo horseedaysa hydrolysis-ka phenylgalactoside iyo sameynta midab buluug ah.

Nadiifinta Borotiinka: Xaaladaha qaarkood, phenylgalactoside waxaa loo isticmaali karaa sidii ligand loogu talagalay koromatografiga isku-xidhka si loo nadiifiyo borotiinnada si gaar ah ugu xiran ama ay hawlgeliyaan β-galactosidase. Borotiinka xiisaha leh wuxuu yeelan karaa calaamad isku-xidhka ama calaamad isku-xidhka oo ka kooban domain isku xidhka β-galactosidase. Marka la gudbiyo isku-darka borotiinka iyada oo loo marayo tiir leh phenylgalactoside aan dhaqdhaqaaq lahayn, borotiinka la rabo si xulasho leh ayaa loo hayn karaa ka dibna waa la soo saari karaa.