4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2

Ogaanshaha dhaqdhaqaaqa β-glucuronidase: 4-NPBG waxaa badanaa loo isticmaalaa substrate chromogenic ah si loo qiimeeyo joogitaanka iyo dhaqdhaqaaqa β-glucuronidase ee muunado kala duwan oo bayooloji ah. Ensaymku wuxuu kala gooyaa isku xidhka glycosidic ee 4-NPBG, isagoo soo saaraya 4-nitrophenol, kaas oo lagu cabbiri karo iyadoo la adeegsanayo spectrophotometry.

Daraasadaha dheef-shiid kiimikaadka daroogada: Maadaama β-glucuronidase uu door muhiim ah ka ciyaaro dheef-shiid kiimikaadka daawooyinka iyo xenobiotics-ka, 4-NPBG waxaa loo isticmaali karaa in lagu qiimeeyo dhaqdhaqaaqa enzyme-kan daraasadaha dheef-shiid kiimikaadka daroogada. Waxay ka caawisaa fahamka baaxadda iyo dhaqdhaqaaqa falgallada glucuronidation, kuwaas oo muhiim u ah nadiifinta daawada iyo helitaanka bioavailability.

Daraasadaha sunta: Qaar ka mid ah iskudhisyada sunta ah ayaa la dheefshiidi karaa oo laga soo saari karaa qaab isku-xidhka glucuronide. Iyagoo u adeegsanaya 4-NPBG substrate ahaan, cilmi-baarayaashu waxay baari karaan dhaqdhaqaaqa β-glucuronidase ee unugyada kala duwan ama khadadka unugyada si ay u qiimeeyaan sunta suurtagalka ah ama saameynta xun ee isku-dhisyada.

Baadhista Kliinikada: Cabbiraadda dhaqdhaqaaqa β-glucuronidase iyadoo la adeegsanayo 4-NPBG waxaa loo isticmaali karaa goobaha caafimaadka si loo ogaado cudurrada ama xaaladaha qaarkood. Heerarka aan caadiga ahayn ama dhaqdhaqaaqa β-glucuronidase waxay muujin karaan cillado hidde ah oo gaar ah, cillad beerka ah, ama kansar.