4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Substrates-ka Ensaym-ka: 4NPM waxaa loo isticmaali karaa substrate ahaan enzymes-ka kala duwan, oo ay ku jiraan glycosidases iyo enzymes-ka la xiriira. Ensaymyadani waxay kala gooyaan xiriirka glycosidic ee u dhexeeya mannose iyo 4NPM, taasoo keenta sii deynta qaybta nitrophenyl. Heerka hydrolysis-ka substrate-ka waxaa lagu cabbiri karaa spectrophotometry iyadoo la kormeerayo nuugista kooxda nitrophenyl-ka ee la sii daayay mowjad gaar ah. Tani waxay u oggolaanaysaa cilmi-baarayaasha inay qiimeeyaan dhaqdhaqaaqa ensaym-ka iyo kinetics-ka.

Tijaabooyin ku saabsan dheef-shiid kiimikaadka karbohaydraytka: Iyagoo u adeegsanaya 4NPM substrate ahaan, cilmi-baarayaashu waxay baran karaan dhaqdhaqaaqa enzymes-ka ku lug leh dheef-shiid kiimikaadka karbohaydraytka, sida alpha-mannosidases. Ensaymadani waxay hydrolyzes ku sameeyaan isku-xidhka glycosidic ee ku jira isku-dhafka mannose-ka, hawlahoodana waxaa lagu cabbiri karaa iyadoo la kormeerayo sii deynta qaybta nitrophenyl ee 4NPM.

Daraasadaha Glycosylation: 4NPM waxaa sidoo kale loo isticmaali karaa baaritaanno si loo baaro ensaymada ku lug leh hababka glycosylation. Glycosylation waa habka lagu xiro molecules sonkorta borotiinada ama molecules kale, ensaymo badanna way ku lug leeyihiin habkan. Iyagoo u adeegsanaya 4NPM substrate aqbale ah, cilmi-baarayaashu waxay cabbiri karaan wareejinta qaybta sonkorta 4NPM iyadoo la adeegsanayo ensaymo gaar ah oo ku lug leh falcelinta glycosylation.

Baaritaanka ka hortagga ensaymka ama firfircoonayaasha: 4NPM waxaa loo isticmaali karaa baaritaannada baaritaanka ee wax-soo-saarka sare leh si loo aqoonsado isku-dhafka joojiya ama dhaqaajiya ensaymyada gaarka ah. Iyadoo la qiimeynayo saameynta isku-dhafka tijaabada ee ku aaddan hydrolysis ama wax-ka-beddelka 4NPM ee ensaymyada bartilmaameedka ah, cilmi-baarayaashu waxay aqoonsan karaan wakiilada daaweynta ee suurtagalka ah ama baaritaannada kiimikada ee waxtarka leh si loo barto shaqada ensaymka.