4-Methylumbelliferyl-beta-D-glucopyranoside CAS: 18997-57-4

Saamaynta 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) waa inay u adeegto sidii substrate-ka beta-glucosidase enzyme.Enzyme Tani waxay jebisaa isku-xidhka glucosidic ee MUG, taasoo keentay in la sii daayo 4-Methylumbelliferone (4-MU) .Codsiga MUG wuxuu ugu horreyntii ku jiraa maadada microbiology, gaar ahaan ogaanshaha iyo aqoonsiga bakteeriyada beta-glucosidase soo saarta.MUG waxaa caadi ahaan loo isticmaalaa ogaanshaha Escherichia coli (E. coli) ee biyaha iyo muunadaha cuntada.E. coli waxay leedahay enzyme beta-glucosidase, kaas oo hydrolyze kara MUG oo soo saari kara calaamada fluorescent ee joogitaanka iftiinka ultraviolet (UV).Marka substrate MUG la nadiifiyo, 4-MU waxay soo saartaa iftiin buluug ah, fududaynta aqoonsiga bakteeriyada leh waxqabadka beta-glucosidase.Habkan waxaa caadi ahaan loo adeegsadaa la socodka deegaanka iyo baaritaanka badbaadada cuntada, maadaama ay bixiso hab degdeg ah oo xasaasi ah oo lagu ogaanayo wasakhowga bakteeriyada.Marka lagu daro codsigeeda microbiology, MUG sidoo kale waxaa loo isticmaali karaa in lagu barto dhaqdhaqaaqa iyo xannibaadda beta-glucosidase Cilmi-baadhista bayoolaji iyo molecular.Fluorescence waxay awood u siinaysaa cabbirka kinetics-ka enzyme waxaana loo isticmaali karaa in lagu baaro horjoogayaasha suurtagalka ah ama firfircoonayaasha waxqabadka beta-glucosidase.Guud ahaan, MUG waa xarun isku dhafan oo ka hela codsi baahsan oo ku saabsan maadooyinka microbiology, biochemistry, iyo biology molecular si loo ogaado ee dhaqdhaqaaqa beta-glucosidase iyo aqoonsiga bakteeriyada soo saarta enzyme this.