Proteinase K oo loogu talagalay albamka tritirachium CAS:39450-01-6

Proteinase K waa ensaym laga soo saaro albamka fangaska Tritirachium. Waa protease serine oo si gaar ah u gaar ah, taasoo la macno ah inay kala jari karto isku xidhka peptide ee borotiinno kala duwan. Proteinase K waxaa loo yaqaanaa awooddeeda ay si hufan u burburin karto borotiinnada, taasoo ka dhigaysa qalab qiimo leh oo loogu talagalay codsiyada kala duwan.

Mid ka mid ah codsiyada ugu muhiimsan ee Proteinase K waa cilmi-baarista bayoolajiga molecular-ka. Waxaa badanaa loo isticmaalaa hab-raacyada soo saarista DNA-da iyo RNA si looga saaro borotiinnada faragelin kara falanqaynta hoose. Proteinase K wuxuu ka caawiyaa nuugista unugyada iyo hoos u dhigista borotiinnada unugyada, taasoo u oggolaanaysa go'doominta asiidhyada nucleic-ga ee tayada sare leh.

Proteinase K sidoo kale si weyn ayaa loogu isticmaalaa falanqaynta borotiinka iyo daraasadaha astaamaha. Waxaa loo isticmaali karaa dheefshiidka borotiinka, taasoo u oggolaanaysa soo saarista jajabyada peptide-ka kuwaas oo lagu falanqeyn karo farsamooyinka sida spectrometry-ga cufka. Intaa waxaa dheer, Proteinase K waxay faa'iido u leedahay isku xigxiga borotiinka iyo aqoonsashada booska amino acids-ka ee silsiladda polypeptide.

Adeegsi kale oo muhiim ah oo Proteinase K ah waa nadiifinta dusha sare. Waxaa si caadi ah loogu isticmaalaa shaybaarrada bayoolajiga molecular-ka si looga saaro asiidhyada nucleic-ga, borotiinnada, iyo wasakhda kale ee qalabka iyo dusha sare ee shaybaarka. Proteinase K waa mid aad u deggan oo firfircoon xaalado kala duwan, oo ay ku jiraan heerkul sare iyo heerarka pH ee kala duwan.