5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide waa iskudhis loo isticmaalo daraasaadka biochemical ee kala duwan, gaar ahaan ogaanshaha iyo aragga ee hawlaha enzyme.Waa substrate-ka lagu nadiifin karo enzymes gaar ah, taasoo keentay in la sii daayo badeecad midab leh ama fluorescent ah.
Xaruntan waxaa caadi ahaan loo isticmaalaa baaritaannada si loo ogaado joogitaanka iyo dhaqdhaqaaqa enzymes sida beta-galactosidase iyo beta-glucuronidase.Enzymes-yadani waxay ka gooyaan kooxaha acetyl iyo glucosaminide substrate, taasoo horseedaysa samaynta koromophore buluug ama cagaar ah.
Qaab dhismeedka gaarka ah ee 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide waxay u oggolaaneysaa in si sahlan loo ogaado iyo qiyaasidda dhaqdhaqaaqa enzyme.Isticmaalkeeda farsamooyin tijaabo ah oo kala duwan, oo ay ku jiraan histochemistry, immunohistochemistry, iyo imtixaannada unugyada ku salaysan, ayaa gacan ka geystay faham wanaagsan oo ku saabsan hawlaha enzyme.