PMSF CAS:329-98-6 Qiimaha Soo-saaraha

Saameyn:

PMSF waa daawo ka hortagta serine protease oo si aan dib u soo kabasho lahayn ugu xidha goobta firfircoon ee serine proteases-ka, taasoo xannibaysa dhaqdhaqaaqooda.

Waxay wax ka beddeshaa haraaga serine-ka iyada oo loo marayo isku-xidhka covalent, taasoo horseedaysa in enzimku aanu shaqayn.

PMSF waxay ka ilaalin kartaa borotiinada inay burburaan iyagoo joojinaya dhaqdhaqaaqa borotiinka, gaar ahaan borotiinka serine.

Codsiyada:

Cilmi-baarista bayoolojiga: PMSF waxaa badanaa loo isticmaalaa inta lagu jiro nadiifinta borotiinka iyo hab-raacyada go'doominta si looga hortago burburka borotiinka. Waxay gacan ka geysataa ilaalinta hufnaanta iyo xasilloonida borotiinka bartilmaameedka ah.

Ka-hortagga Ensaymka: PMSF waa fal-celiye lagama maarmaan u ah joojinta borotiinnada serine inta lagu jiro tijaabooyinka ensaymka ama daraasadaha bayoolaji. Iyagoo xannibaya dhaqdhaqaaqa borotiinnadan, cilmi-baarayaashu waxay baari karaan doorka borotiinnada serine ee hababka kala duwan ee bayoolojiga.

Daraasadaha Proteolysis: PMSF waxaa loo isticmaali karaa in lagu xakameeyo ama lagu hagaajiyo dhaqdhaqaaqa protease ee lysates-ka unugyada ama soosaarka. Tani waxay faa'iido u leedahay barashada dhacdooyinka proteolytic ee ku lug leh hababka unugyada ama marka proteolysis loo baahan yahay in lagu xakameeyo dejinta tijaabada.

Joojinta guud ee protease: In kasta oo PMSF si gaar ah u bartilmaameedsato protease-ka serine, haddana waxay sidoo kale yeelan kartaa saameyn xakameyn ah oo ku saabsan qaar ka mid ah protease-ka cysteine. Waxaa loo isticmaalaa sidii joojiye guud oo protease ah marka lala shaqeynayo muunadaha borotiinka si loo yareeyo burburka protease-ka.