P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Ogaanshaha dhaqdhaqaaqa beta-galactosidase: PNGG waxaa badanaa loo isticmaalaa baaritaannada si loo cabbiro dhaqdhaqaaqa beta-galactosidase, oo ah ensaym kiciya hydrolysis-ka lactose una beddela gulukoos iyo galactose. Hydrolysis-ka PNGG ee beta-galactosidase wuxuu sii daayaa molecule p-nitrophenol (pNP), kaas oo lagu ogaan karo spectrophotometric sababtoo ah midabkiisa jaalaha ah.

Baaritaanka daawooyinka ka hortaga iyo firfircoonida enzyme-ka: PNPG waxaa loo isticmaali karaa baaritaanka sare si loo aqoonsado walxaha wax ka beddela dhaqdhaqaaqa beta-galactosidase. Iyadoo la cabbirayo heerka hydrolysis-ka PNGG iyadoo ay jiraan walxo tijaabo ah oo kala duwan, cilmi-baarayaashu waxay aqoonsan karaan daawooyinka ka hortaga ee yareeya dhaqdhaqaaqa enzyme-ka ama kuwa kiciya ee kor u qaada dhaqdhaqaaqa enzyme-ka.

Daraasad ku saabsan kinetics-ka ensaymka: Hydrolysis-ka PNGG ee beta-galactosidase wuxuu raacaa kinetics-ka Michaelis-Menten, taasoo u oggolaanaysa cilmi-baarayaasha inay go'aamiyaan xuduudaha ensaymka muhiimka ah sida xawaaraha falcelinta ugu badan (Vmax) iyo joogtada Michaelis (Km). Macluumaadkani wuxuu ka caawiyaa fahamka isku xirnaanta substrate-ka ensaymka iyo hufnaanta katalytic-ga.

Codsiyada bayoolajiga molecular-ka: Beta-galactosidase, oo kala gooya PNPG, waxaa badanaa loo isticmaalaa sidii hiddo-wade ku takhasusay bayoolajiga molecular-ka. Substrate-ka PNPG waxaa badanaa loo isticmaalaa in lagu ogaado oo lagu sawiro muujinta hiddo-wadaha wariyaha, taasoo bixinaysa hab fudud oo xasaasi ah oo lagu qiimeeyo muujinta hiddo-wadaha ee nidaamyada tijaabada ee kala duwan.