P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Ogaanshaha dhaqdhaqaaqa beta-galactosidase: PNPG waxaa caadi ahaan loo isticmaalaa baaritaannada si loo cabbiro dhaqdhaqaaqa beta-galactosidase, ensaymes oo kataliya hydrolysis ee lactose galay glucose iyo galactose.Hydrolysis of PNPG by beta-galactosidase ayaa sii daaya p-nitrophenol (pNP) molecule, kaas oo lagu ogaan karo spectrophotometrically sababtoo ah midabkiisa jaalaha ah.

Baadhitaanka enzyme-ka-horjoogayaasha iyo hawl-wadeennada: PNPG waxaa loo isticmaali karaa baadhista sare-u-qaadista si loo aqoonsado isku-dhisyada beddela waxqabadka beta-galactosidase.Iyadoo la cabbirayo heerka PNPG hydrolysis ee joogitaanka xeryahooda tijaabada ee kala duwan, cilmi-baarayaashu waxay aqoonsan karaan xannibaadayaasha hoos u dhigaya dhaqdhaqaaqa enzyme ama firfircoonayaasha kuwaas oo kor u qaadaya dhaqdhaqaaqa enzyme.

Daraasada kinetics-ka enzyme: Hydrolysis of PNPG by beta-galactosidase waxay raacdaa Michaelis-Menten kinetics, taasoo u oggolaanaysa cilmi-baarayaasha inay go'aamiyaan xuduudaha enzyme muhiimka ah sida xawaaraha falcelinta ugu badan (Vmax) iyo Michaelis joogto ah (Km).Macluumaadkani waxa uu gacan ka geystaa fahamka xidhiidhka substrate-ka enzyme-ka iyo waxtarka catalytic.

Codsiyada bayoolojiga molecular: Beta-galactosidase, kaas oo jebiya PNPG, ayaa caadi ahaan loo adeegsadaa hidda-wadaha wariyaha ee bayoolaji molecular.Substrate-ka PNPG waxaa badanaa loo isticmaalaa in lagu ogaado oo lagu sawiro muujinta hidda-wadaha weriyaha, iyada oo siinaya hab fudud oo xasaasi ah oo lagu qiimeeyo muujinta hidda-wadaha ee hababka tijaabada ah ee kala duwan.