HEPES-Na CAS: 75277-39-3 Qiimaha soo saaraha

Wakiilka kaydinta: HEPES sodium cusbada waxaa caadi ahaan loo isticmaalaa sidii wakiilka kaydiya ee tijaabooyinka bayooloji iyo bayooloji.Waxay ka caawisaa in la ilaaliyo kala duwanaanta pH, gaar ahaan marka loo eego kala duwanaanshaha jireed (pH 7.2-7.6).Awooddeeda kaydintu waxay ka dhigaysaa mid qiimo leh ilaalinta xaaladaha ku habboon falcelinta enzymatic ee kala duwan, dhaqanka unugyada, iyo farsamooyinka bayoloji kellika ah.

Dhaqanka unugga: Cusbada soodhiyamka ee HEPES waxa si aad ah loogu isticmaalaa sidii wakiil difaac u ah warbaahinta dhaqanka unugga.Awoodda lagu ilaalinayo pH xasilloon ayaa lagama maarmaan u ah koritaanka saxda ah iyo jiritaanka unugyada.HEPES ayaa inta badan laga doorbidaa wakiilada kale ee wax-dhiseeya maadaama aysan muujin isbedel weyn oo pH ah marka ay la kulmaan jawiga hawada CO2.

Daraasadaha Enzyme: cusbada soodhiyamka ee HEPES waxay si gaar ah faa'iido u leedahay daraasadaha enzymatic halkaas oo loo baahan yahay jawi pH joogto ah oo la kantaroolo.Waxay ka hortagtaa isbeddellada ba'an ee pH inta lagu jiro falcelinta enzymatic, hubinta waxqabadka enzyme ugu fiican.

Electrophoresis: HEPES sodium cusbada waxaa sida caadiga ah loo isticmaalaa sidii wakiilka baffer ee farsamooyinka electrophoretic kala duwan sida polyacrylamide gel electrophoresis (PAGE) iyo agarose gel electrophoresis.Waxay ka caawisaa ilaalinta pH ee kaydiyaha, taas oo muhiim u ah kala-soocidda iyo falanqaynta asiidhka nucleic-ka iyo borotiinnada.

Qiimaynta biochemical: HEPES cusbada soodhiyamka waxaa inta badan lagu isticmaalaa tijaabooyin kala duwan oo bayoolaji ah, oo ay ku jiraan tijaabooyinka enzymes, qiimaynta borotiinka, iyo qiimaynta spectrophotometric.Waxay ka caawisaa in la ilaaliyo qiyaasta pH ee la rabo ee loo baahan yahay natiijooyinka saxda ah iyo kuwa la soo saari karo.

Samaynta daawada: HEPES sodium cusbada sidoo kale waxaa loo isticmaalaa habaynta dawooyinka sida wakiilka kaydinta si loo dejiyo qaabaynta daawada loona ilaaliyo qiyaasta pH ee la doonayo.