FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

Fluorescein mono-beta-D-galactopyranoside, oo sidoo kale loo yaqaan FMG, waa unug ka samaysan fluorescent oo caadi ahaan loo isticmaalo sidii substrate ee tijaabooyinka bayoolajiga unugyada kala duwan.Waxaa laga soo qaatay methyl-beta-D-galactopyranoside iyadoo lagu xirayo molecule fluorescein ah.FMG waxaa si weyn loo isticmaalaa in lagu barto dhaqdhaqaaqa beta-galactosidase, enzim kaas oo kicinaya hydrolysis ee lactose galay galactose iyo glucose.Adigoo isticmaalaya FMG substrate ahaan, cilmi-baarayaashu waxay la socon karaan dhaqdhaqaaqa enzymatic ee beta-galactosidase iyada oo loo marayo cabbirka qiiqa qiiqa.Hydrolysis of FMG by beta-galactosidase waxay keenaysaa in la sii daayo fluorescein, taasoo keentay in kororka signal fluorescent ah oo la qiyaasi karo. Xaruntan waxa kale oo loo isticmaalaa in lagu baaro aqoonsiga karbohaydrayt iyo isdhexgalka.FMG waxaa loo isticmaali karaa baaritaan molecular si loo daraaseeyo isku xirnaanta lectins-ka (borootiinnada si gaar ah ugu xira karbohaydraytyada) karbohaydraytyada ka kooban galactose.Isku-xidhka isku-xidhka FMG-lectin waa la ogaan karaa oo la qiyaasi karaa iyadoo lagu saleynayo isbeddelada qiiqa qiiqa. Guud ahaan, FMG waa qalab wax ku ool ah oo lagu baranayo dhaqdhaqaaqa enzyme iyo aqoonsiga karbohaydrayt, oo bixiya hab ku habboon oo xasaasi ah si loo cabbiro fluorescence loona qiimeeyo hababkan noolaha.

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Fluorescein mono-beta-D-galactopyranoside (FMG) waa unug caadi ah oo loo isticmaalo cilmi-baarista bayoolojiga sida substrate si loo ogaado joogitaanka iyo dhaqdhaqaaqa beta-galactosidase enzyme.FMG waa nooc ka mid ah lactose-ka sokorta waxaana lagu daraa molecule fluorescein ah.

Saamaynta ugu weyn ee FMG waa in ay si gaar ah hydrolyzed by beta-galactosidase, enzim ah oo jebiya lactose galay galactose iyo glucose.Hydrolysis-ka enzymatic ee FMG wuxuu horseedaa sii deynta fluorescein, kaas oo siidaaya calaamad xoog leh.

Codsiga aasaasiga ah ee FMG waa ogaanshaha iyo cabbiridda dhaqdhaqaaqa beta-galactosidase ee muunado kala duwan.Insaymiskan waxaa laga helaa noole badan, oo ay ku jiraan bakteeriyada iyo unugyada naasleyda, shaqadeeduna waxay muujin kartaa hababka kala duwan ee unugyada iyo dariiqyada dheef-shiid kiimikaadka.

Adigoo isticmaalaya FMG substrate ahaan, beta-galactosidase dhaqdhaqaaqa waxaa lagu qiyaasi karaa iyadoo la kormeerayo fluorescence ka soo baxa fluorescein la xoreeyay.Cabbirkan waxaa lagu samayn karaa qaabab tijaabo oo kala duwan, oo ay ku jiraan baaritaannada vitro iyo daraasadaha sawirka unugyada nool.

Intaa waxaa dheer, FMG waxaa loo isticmaali karaa sidii qalab lagu daraaseeyo qaybinta iyo meelaynta beta-galactosidase gudaha unugyada.Iyadoo la adeegsanayo farsamooyinka muraayada indhaha, cilmi-baarayaashu waxay arki karaan fluorescence ka soo baxa FMG marka loo eego hydrolysis, iyaga oo u oggolaanaya inay la socdaan dhaqdhaqaaqa booska iyo ku-meel-gaadhka ah ee beta-galactosidase.

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Halabuurka	C26H22O10
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CAS Maya.	102286-67-9
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