Fluorescein Isothiocyante CAS:3326-32-7

Sifooyinka Iftiinka: FITC waxay leedahay wax soo saar badan oo kutaan ah waxayna soo saartaa iftiin cagaaran oo dhalaalaya marka la kiciyo qiyaastii 488 nm. Sifooyinka iftiinka waxay ka dhigayaan mid ku habboon ogaanshaha iyadoo la adeegsanayo farsamooyin sawireed oo kala duwan, sida mikroskoobka iftiinka, socodka cytometry, iyo spectroscopy-ga iftiinka.

Isku xidhka Covalent: FITC waxay ka kooban tahay koox isothiocyanate ah oo u oggolaanaysa inay si isku xiran ugu xidho kooxaha amino ee ku jira borotiinnada, unugyada difaaca jirka, iyo molecules-ka kale ee noolaha. Isku xidhka covalent wuxuu hubiyaa calaamadayn deggan oo gaar ah oo ku saabsan molecules-ka bartilmaameedka ah, taasoo suurtogalinaysa ogaanshaha iyo muuqaalkooda.

Immunofluorescence: Unugyada difaaca jirka ee calaamadaysan ee FITC ayaa si caadi ah loogu isticmaalaa baaritaannada immunofluorescence si loo ogaado antigens ama borotiinno gaar ah oo ku jira unugyada iyo unugyada. Iftiinka cagaaran ee dhalaalaya ee FITC wuxuu u suurtageliyaa cilmi-baarayaasha inay meeleeyaan oo ay sawiraan qaybinta molecules-ka bartilmaameedka ah ee ku jira muunadaha bayoolojiga.

Cytometry-ga Socodka: Unugyada difaaca jirka ee calaamadaysan ee FITC ama biomolecules-ka kale ee fluorochrome-conjugated ayaa si weyn loogu isticmaalaa cytometry-ga socodka si loo falanqeeyo loona kala saaro unugyada iyadoo lagu saleynayo calaamadaha dusha sare ama qaybaha gudaha unugyada. FITC waxay la jaanqaadaysaa laysarka 488 nm ee si caadi ah loo isticmaalo ee loo isticmaalo qalabka cytometry-ga socodka.

Daraasadaha isdhexgalka borotiinka: FITC waxaa loo isticmaali karaa daraasadaha isdhexgalka borotiinka-borotiin si loo qiimeeyo isku xirnaanta iyo isdhexgalka borotiinada. Iyagoo calaamadeynaya borotiinno gaar ah FITC, cilmi-baarayaashu waxay falanqeyn karaan isdhexgalka iyaga oo adeegsanaya farsamooyin sida wareejinta tamarta resonance fluorescence (FRET) ama polarization fluorescence.

Dabagalka molecule-ka noolaha: FITC waxaa loo isticmaali karaa in lagu raad raaco oo lagu dabagalo dhaqdhaqaaqa molecule-yada noolaha ee ku jira unugyada nool ama unugyada. Iyagoo calaamadeynaya molecules-ka xiisaha leh ee FITC, cilmi-baarayaashu waxay baran karaan deegaankooda, dhaqdhaqaaqyadooda, iyo qaababka ka ganacsiga waqtiga dhabta ah iyagoo adeegsanaya mikroskoobka fluorescence.

Codsiyada ogaanshaha: Baaritaannada ama unugyada difaaca jirka ee lagu calaamadeeyay FITC ayaa loo isticmaalaa ogaanshaha noocyada kala duwan, oo ay ku jiraan ogaanshaha antigens gaar ah, wakiilada faafa, ama biomarkers ee muunadaha bukaanka. Baaritaannadan fluorescent-ka ah waxay bixiyaan ogaansho xasaasi ah oo gaar ah, taasoo u oggolaanaysa ogaanshaha degdega ah oo sax ah.