Ethidium Bromide CAS:1239-45-8

Ethidium bromide (EtBr) waa midab fluorescent caadi ah oo loo isticmaalo bayoolajiga molecular iyo bayochemistry si loo arko asiidhyada nucleic, gaar ahaan DNA, ee ku jira agarose gel electrophoresis. Waxay leedahay xiriir sare oo DNA ah, marka la soo bandhigo iftiinka ultraviolet (UV), fluoresces-ka wuxuu soo saaraa midab casaan-liimi ah. Tani waxay u oggolaanaysaa cilmi-baarayaasha inay si fudud u ogaadaan oo ay u falanqeeyaan jajabyada DNA-da ee loo kala saaray cabbirka matrix jel.

EtBr wuxuu dhex maraa lammaanayaasha salka ee DNA iyo RNA, taasoo keenta isbeddel ku yimaada sifooyinka iftiinka. Badanaa waxaa lagu daraa jellada agarose ama waxaa lagu qasaa muunadaha DNA ka hor electrophoresis. Marka jelku la kulmo iftiinka UV, xargaha DNA-da ee ka kooban EtBr waxay u muuqdaan sidii xargaha oranji dhalaalaya oo ka soo horjeeda asalka mugdiga ah, taasoo sahlaysa in la arko oo la falanqeeyo jajabyada DNA-da.

Dalbo ama Codso Qiimayn

Faahfaahinta Badeecada

Calaamadaha Alaabta

Codsiga iyo Saamaynta

Muuqaalaynta DNA/RNA: EtBr waxaa inta badan loo isticmaalaa in lagu sawiro DNA iyo RNA marka la isticmaalayo electrophoresis jel agarose. Waxay isku xidhaa lammaanayaasha salka ee molecules-ka asiidhka nukliyeerku, taasoo keenta inay dhalaalaan marka la kulmo iftiinka UV. Tani waxay u oggolaanaysaa cilmi-baarayaasha inay sawiraan oo ay falanqeeyaan kala-soocidda jajabyada DNA ama RNA iyadoo lagu salaynayo cabbirkooda ama isku xigxigooda.

Wasakhaynta DNA-da: EtBr waxaa loo isticmaali karaa in lagu wasakheeyo DNA-da habab kala duwan oo tijaabo ah. Waxaa lagu dari karaa muunado DNA ah, sida plasmids-ka la safeeyey ama alaabada PCR, si loo xaqiijiyo joogitaankooda iyo xooggooda. DNA-da la wasakheeyey ayaa markaa lagu arki karaa iyadoo la adeegsanayo iftiinka UV ama nidaamka sawir-qaadista jel.

Tirakoobka DNA-da: EtBr waxaa loo isticmaali karaa in lagu cabbiro heerka DNA-da. Marka la barbardhigo xoogga xargaha DNA-da ee EtBr-ka ee ku jira jelka agarose iyo heerarka la yaqaan ee heerka DNA-da, waxaa la go'aamin karaa qiyaasta qiyaasta DNA-da ee muunad.

Microskoobka Iftiiminta: EtBr waxaa loo isticmaali karaa mikroskoobka fluorescence si loo wasakheeyo loona arko DNA-da unugyada nool ama unugyada. Waxay u oggolaanaysaa cilmi-baarayaasha inay bartaan deegaanka DNA-da iyo dhaqdhaqaaqa unugyada ama ay daawadaan kala-goynta DNA-da ee unugyada apoptotic-ga.

Baakaynta Alaabta:

Macluumaad Dheeraad ah:

Halabuurka	C21H20BrN3
Baaritaan	99%
Muuqaalka	Budada casaan ilaa guduud mugdi ah
Lambarka CAS.	1239-45-8
Baakad	Yar iyo weyn
Muddada Khafiska	2 sano
Kaydinta	Ku kaydi meel qabow oo qalalan
Shahaadada	ISO.