DEPC CAS:1609-47-8 Qiimaha Soo-saaraha

Diethyl pyrocarbonate (DEPC) waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo shaybaarrada bayoolajiga moolikuyuullada. Waa dareere cad oo aan midab lahayn oo si aad ah uga falceliya isku-dhisyada nucleophilic sida amines iyo thiols. DEPC waxaa ugu horreyn loo isticmaalaa in lagu joojiyo RNases (ribonucleases), ensaymyada burburin kara RNA, iyadoo wax laga beddelayo goobahooda firfircoon. Waxay tan ku gaartaa iyadoo la falgasha haraaga histidine ee ensaymka, kuwaas oo lagama maarmaan u ah waxqabadkiisa. Tani waxay ka dhigaysaa DEPC qalab qiimo leh oo ku jira tijaabooyinka la xiriira RNA waxayna hubineysaa ilaalinta sharafta RNA. Marka lagu daro sifooyinkeeda joojiya RNase, DEPC waxay sidoo kale wax ka beddeli kartaa molecules-ka kale ee noolaha, sida borotiinnada iyo DNA-da, sababtoo ah falcelinteeda kooxaha nucleophilic. Taxaddar waa in la sameeyaa marka la maareynayo DEPC, maadaama ay tahay sun oo ay dhici karto inay isbeddelaan.

Dalbo ama Codso Qiimayn

Faahfaahinta Badeecada

Calaamadaha Alaabta

Codsiga iyo Saamaynta

Joojinta RNases: Ujeeddada ugu weyn ee DEPC waa in la joojiyo RNases, kuwaas oo ah ensaymo burburin kara RNA. DEPC waxay la falgashaa haraaga histidine ee RNases, iyadoo wax ka beddesha goobahooda firfircoon oo ka dhigaysa kuwo aan firfircoonayn. Tani waxay hubinaysaa ilaalinta sharafta RNA inta lagu jiro tijaabooyinka sida go'doominta RNA, qoraalka dib u celinta, iyo taxanaha RNA.

Ilaalinta muunadaha RNA: Daaweynta DEPC waxaa badanaa lagu dabaqaa qalabka shaybaarka, sida tuubooyinka microcentrifuge, cidhifyada pipette-ka, iyo biyaha loo isticmaalo tijaabooyinka RNA. Tani waxay gacan ka geysaneysaa in la joojiyo wixii RNANases ah ee harsan ee laga yaabo inay jiraan iyo in laga hortago burburka RNA inta lagu jiro maaraynta iyo kaydinta muunadaha.

Diyaarinta reagent-ka aan RNAase lahayn: DEPC waxaa loo isticmaali karaa in lagu daaweeyo xalalka ama reagent-yada u baahan in aan RNAase lahayn, sida buffers-yada, xalalka Tris-EDTA (TE), ama biyaha loo isticmaalo tijaabooyinka bayoolajiga molecular-ka. Xalalkan la daweeyay ayaa markaa si ammaan ah loogu isticmaali karaa si loo yareeyo burburka RNA.

Wax-ka-beddelka DNA-da: DEPC waxay sidoo kale la falgeli kartaa kooxaha amino ee ku jira molecules-ka DNA-da, taasoo keenta wax-ka-beddelka carboethylated ee saldhigyada DNA-da. Tan waxaa loo isticmaali karaa codsiyada gaarka ah, sida fal-celinta taxanaha DNA-da ama wax-ka-beddelka DNA-da ee daraasadaha isku-xidhka.

Baakaynta Alaabta:

Macluumaad Dheeraad ah:

Halabuurka	C6H10O5
Baaritaan	99%
Muuqaalka	Dareere aan midab lahayn
Lambarka CAS.	1609-47-8
Baakad	Yar iyo weyn
Muddada Khafiska	2 sano
Kaydinta	Ku kaydi meel qabow oo qalalan
Shahaadada	ISO.