BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine cusbo CAS:6578-06-9

5-Bromo-4-chloro-3-indolyl phosphate cusbada p-toluidine waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo bayoolajiga molecular-ka iyo cilmi-baarista bayochemistry-ga. Waxaa badanaa loo isticmaalaa baaritaannada difaaca jirka ee enzyme-linked immunosorbent (ELISA) iyo farsamooyinka calaamadaynta borotiinka.

Isku-darkani waa substrate loogu talagalay alkaline phosphatase, ensaym si weyn loogu isticmaalo bayoolajiga molecular-ka si loo ogaado jiritaanka molecules ama borotiinno gaar ah iyada oo loo marayo falgallada midabka ama kiimikada.

Marka ensaymka alkaline phosphatase uu jiro, wuxuu kala saaraa kooxaha fosfate-ka cusbada 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine, taasoo keenta in la sameeyo midab buluug ama guduud ah. Isbeddelkan midabka si fudud ayaa loo ogaan karaa oo loo cabbiri karaa, taasoo suurtogalinaysa cabbirka dhaqdhaqaaqa ensaymka ama joogitaanka molecules-ka bartilmaameedka ah.

Dalbo ama Codso Qiimayn

Faahfaahinta Badeecada

Calaamadaha Alaabta

Codsiga iyo Saamaynta

Western blotting: BCIP waxaa loo isticmaalaa in lagu ogaado oo lagu arko borotiinnada loo gudbiyo xuubka ka dib electrophoresis-ka jel. Antibodies-ka labaad ee alkaline phosphatase-conjugated ayaa loo isticmaalaa in lagu xidho unugyada difaaca jirka ee aasaasiga ah, ka dibna ku-shubista BCIP waxay keentaa horumarinta daadad buluug ah oo ku taal goobta ay ku yaalliin unugyada borotiinka.

Immunohistochemistry: BCIP waxaa loo isticmaalaa farsamooyinka rinjiyeynta immunohistochemical si loo ogaado jiritaanka borotiinno gaar ah oo ku jira unugyada. Unugyada difaaca jirka ee alkaline phosphatase-ka isku xiran ayaa loo isticmaalaa in lagu xidho unugyada difaaca jirka ee aasaasiga ah, daaweynta BCIP-na waxay abuurtaa midab buluug ah marka uu jiro ensaym, taasoo muujinaysa in borotiinku uu ku jiro muunadda unugyada.

Isku-dhafka In situ: BCIP waxaa sidoo kale loo isticmaalaa in lagu ogaado taxanaha asiidhka nucleic ee unugyada go'an ama unugyada. Baaritaannada isku-dhafka waxaa lagu calaamadeeyaa phosphatase alkaline, marka lagu daro BCIP, joogitaanka mRNA ama DNA-da bartilmaameedka ah waxaa lagu muujiyaa horumarinta midab buluug ah.

Baakaynta Alaabta:

Macluumaad Dheeraad ah:

Halabuurka	C8H6BrClNO4P.C7H9N
Baaritaan	99%
Muuqaalka	Budada cad
Lambarka CAS.	6578-06-9
Baakad	Yar iyo weyn
Muddada Khafiska	2 sano
Kaydinta	Ku kaydi meel qabow oo qalalan
Shahaadada	ISO.