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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium cusbo CAS:129541-41-9

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium cusbada waa iskudhis kiimiko ah oo caadi ahaan loo isticmaalo cilmi-baarista shaybaarka iyo ogaanshaha.Inta badan waxaa loo yaqaan X-Gluc waxaana si ballaaran loo isticmaalaa sidii substrate loogu talagalay ogaanshaha waxqabadka enzyme beta-glucuronidase.

Marka beta-glucuronidase ay jirto, waxay jebisaa dammaanadda glucuronide ee X-Gluc, taasoo keentay in la xoreeyo midab buluug ah oo loo yaqaan 5-bromo-4-chloro-3-indolyl.Dareen-celintan waxaa caadi ahaan loo isticmaalaa in muuqaal ahaan ama spectrophotometrically lagu ogaado muujinta beta-glucuronidase enzyme ee unugyada ama unugyada.

Nooca cusbada sodium ee X-Gluc waxay wanaajisaa milmaheeda xalalka aqueous, fududaynta isticmaalka shaybaarka.X-Gluc waxaa inta badan loo adeegsadaa cilmi-baarista bayoolajiga molecular si loo barto muujinta hidda-wadaha, dhaqdhaqaaqa dhiirrigeliyaha, iyo qiimaynta hidda-wadaha wariyaha.Waxa kale oo loo isticmaali karaa in lagu ogaado joogitaanka beta-glucuronidase noolaha soo saara, sida bakteeriyada qaarkood, ee daraasaadka microbiological.


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Ogaanshaha GUS: X-Gluc waxaa kala jeexjeexay ensaymka GUS oo galay ka samaysan buluug aan milmi karin oo loo yaqaan 5-bromo-4-chloro-3-indole (X-Ind).Dareen-celintani waxay u oggolaanaysaa aragtida iyo qiyaasidda dhaqdhaqaaqa GUS ee unugyada iyo unugyada.

Daraasadaha muujinta hidda-wadaha: X-Gluc waxaa loo adeegsadaa unugga wariyaha ee daraasadaha muujinta hidda-wadaha.Marka la isku daro hidda-wadaha GUS ee dhiirrigeliyaha xiisaha, cilmi-baarayaashu waxay go'aamin karaan dhaqdhaqaaqa iyo qaabka ku-meel-gaadhka ah ee dhiirrigeliyaha iyada oo loo marayo ogaanshaha dhaqdhaqaaqa GUS iyadoo la adeegsanayo X-Gluc.

Falanqaynta dhirta transgenic: Nidaamka hidda-wadaha wariyaha ee GUS ayaa si weyn loogu isticmaalaa bayoolaji molecular dhirta.Midabaynta X-Gluc waxay u ogolaataa cilmi-baarayaasha inay ogaadaan oo bartaan qaababka muujinta transgene-ka ee dhirta.Tani waxay gacan ka geysataa fahamka nidaaminta hidda-socodka, muujinta unugyada gaarka ah, iyo bayoolajiga korriinka ee dhirta.

Injineernimada hidaha: X-Gluc waxaa loo isticmaalaa calaamad la dooran karo ee tijaabooyinka injineernimada hidaha.Marka la isku xiro hidda-wadaha GUS ee hiddaha shisheeye ee xiisaha leh, midabaynta X-Gluc waxaa loo isticmaali karaa in lagu aqoonsado isbeddelka guusha leh iyo isdhexgalka hiddo-wadaha la rabo ee noolaha.

Cilmi-baarista Microbiology: X-Gluc waxaa loo isticmaali karaa in lagu ogaado oo lagu garto bakteeriyada GUS soo saarta.Enzyme GUS waxaa laga helaa noocyo badan oo kala duwan oo bakteeriyada ah, iyo midabaynta X-Gluc waxay u oggolaaneysaa aragtida iyo aqoonsiga bakteeriyada GUS-positive ee daraasaadka microbiological.

Tusaalaha Alaabta

129541-41-9-2
129541-41-9-3

Xiritaanka alaabta:

6892-68-8-3

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CAS Maya. 129541-41-9
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