5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium cusbo CAS:18656-96-7

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide Cusbo cyclohexylammonium ah, oo sidoo kale loo yaqaan X-glucuronide, waa isku-dhis kiimiko ah oo si caadi ah loogu isticmaalo bayoolajiga molecular-ka iyo tijaabooyinka enzymology-ga. Waa substrate koromogenic ah oo inta badan loo isticmaalo in lagu ogaado dhaqdhaqaaqa enzyme-ka beta-D-glucuronidase.

Isku-darka wuxuu ka kooban yahay koox indole ah oo ku xiran qayb ka mid ah aashitada glucuronic iyada oo loo marayo isku-xidhka glycosidic. Waxyaabaha ku jira bromo iyo chloro waxay bixiyaan xasillooni waxayna xoojiyaan milmida isku-darka.

Marka lagu daro muunad ka kooban beta-D-glucuronidase, ensaymku wuxuu kala gooyaa isku xidhka glycosidic, isagoo sii deynaya qaybta indole. Falgalkani wuxuu keenaa sameynta dhibic buluug ah ama isbeddel midab, taasoo u oggolaanaysa in si fudud loo ogaado dhaqdhaqaaqa ensaymka muuqaal ama muuqaal.

Iyada oo ay ugu wacan tahay gaar ahaanshaha beta-D-glucuronidase, X-glucuronide waxaa si weyn loogu isticmaalaa farsamooyinka bayoolajiga molecular sida baaritaanka bakteeriyada iyo isbeddelka khamiirka, baaritaanka hidda-wadaha ee wariyaha, iyo rinjiyeynta histochemical. Waxay bixisaa hab ku habboon oo xasaasi ah si loo ogaado jiritaanka ama muujinta beta-D-glucuronidase waxaana badanaa loo adeegsadaa iyadoo lala kaashanayo baaritaannada kale ee hidde-sideyaasha ama bayoolajiyada.

Dalbo ama Codso Qiimayn

Faahfaahinta Badeecada

Calaamadaha Alaabta

Codsiga iyo Saamaynta

Saamaynta milixda cyclohexylammonium ee 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide (X-glucuronide) waa awooddeeda ay ku soo saarto isbeddel buluug ah ama midab ah marka ay joogaan ensaymka beta-D-glucuronidase. Falcelinta ensaymkani waxay dhacdaa marka xiriirka glycosidic ee u dhexeeya kooxda indole iyo qaybta aashitada glucuronic uu kala gooyo beta-D-glucuronidase.

Adeegsiga X-glucuronide wuxuu inta badan ku jiraa tijaabooyinka bayoolajiga molecular iyo enzymology. Waxaa badanaa loo isticmaalaa substrate si loo ogaado dhaqdhaqaaqa beta-D-glucuronidase. Qaar ka mid ah codsiyada gaarka ah waxaa ka mid ah:

Baaritaannada hidda-wadaha ee Weriyeyaasha: X-glucuronide waxaa badanaa loo adeegsadaa sidii substrate-ka baaritaanka hidda-wadaha ee weriyeyaasha halkaas oo beta-D-glucuronidase loo isticmaalo sidii enzyme weriye. Iyagoo isku daraya gobolka dhiirrigeliyaha ama ku dhejinaya taxanaha hidda-wadaha xiisaha u leh hidda-wadaha beta-D-glucuronidase, cilmi-baarayaashu waxay cabbiri karaan muujinta ama dhaqdhaqaaqa hidda-wadaha iyagoo la socda sameynta isbeddelka buluugga ah ama isbeddelka midabka marka lagu daro X-glucuronide.

Tijaabooyinka isbeddelka bakteeriyada iyo khamiirka: X-glucuronide waxaa loo isticmaali karaa in lagu ogaado isbeddelka guuleysta ee bakteeriyada ama khamiirka iyadoo la adeegsanayo vector ka kooban hidda-wadaha beta-D-glucuronidase. Unugyada isbeddelay ee muujinaya ensaymka ayaa hydrolyze-ka ku samayn kara X-glucuronide, taasoo soo saari karta isbeddel midab ama soo-dhac muuqda.

Rinjiyeynta Histochemical: X-glucuronide waxaa loo isticmaalaa farsamooyinka rinjiyeynta histochemical si loo arko jiritaanka iyo qaybinta dhaqdhaqaaqa beta-D-glucuronidase ee unugyada ama unugyada. Rinjiyeyntani waxay u oggolaanaysaa meelaynta iyo aqoonsiga unugyada muujinaya ensaymka.

Baakaynta Alaabta:

Macluumaad Dheeraad ah:

Halabuurka	C26H36BrClN2O7
Baaritaan	99%
Muuqaalka	Budada cad
Lambarka CAS.	18656-96-7
Baakad	Yar iyo weyn
Muddada Khafiska	2 sano
Kaydinta	Ku kaydi meel qabow oo qalalan
Shahaadada	ISO.