3,3′-Diaminobenzidine CAS:91-95-2

Saamaynta 3,3'-Diaminobenzidine (DAB) waa inay soo saarto midab bunni ah marka ay la falgasho enzymes-ka peroxidase. Isbeddelkan midabka ayaa u oggolaanaya muuqaalka antigens-ka bartilmaameedka ah ama biomolecules-ka xiisaha u leh immunohistochemistry (IHC) iyo immunocytochemistry (ICC) staining testing. DAB waxaa badanaa loo isticmaalaa cilmi-baarista biomedical-ka iyo codsiyada ogaanshaha si loo ogaado loona meeleeyo antigens-ka gaarka ah ama borotiinnada gudaha muunadaha bayoolojiga. Iyadoo lagu darayo DAB substrate iyo falcelinta xigta ee enzymes-ka peroxidase, soo-saar bunni ah ayaa laga sameeyaa goobta antigens-ka bartilmaameedka ah. Codsiga rinjiyeynta DAB wuxuu si gaar ah waxtar ugu leeyahay barashada cudurro iyo xaalado kala duwan. Waxay u suurtogelinaysaa cilmi-baarayaasha iyo cilmu-nafsiga inay arkaan oo ay falanqeeyaan qaybinta, tirada, iyo deegaanka biomolecules-ka gaarka ah ee unugyada ama unugyada. Macluumaadkani wuxuu ka caawiyaa fahamka hababka cudurrada, barashada hababka unugyada, iyo aqoonsashada bartilmaameedyada ogaanshaha ama daaweynta ee suurtagalka ah. Intaa waxaa dheer, rinjiyeynta DAB waxaa loo isticmaali karaa farsamooyin kala duwan oo molecular ah si loo baaro isdhexgalka borotiinka-borotiinka, loo aqoonsado qaababka muujinta borotiinka, loo go'aamiyo jiritaanka calaamado gaar ah, iyo loo qiimeeyo waxtarka faragelinta daaweynta. Sababtoo ah rinjiyeynta la isku halleyn karo oo adag, iyo sidoo kale iswaafajinta nidaamyada ogaanshaha kala duwan iyo farsamooyinka mikroskoobka, DAB waxay noqotay substrate chromogenic ah oo si ballaaran loo isticmaalo baaritaanka immunohistochemistry iyo immunocytochemistry.