3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

Ogaanshaha dhaqdhaqaaqa beta-galactosidase: ONPG waxaa badanaa loo isticmaalaa si loo go'aamiyo joogitaanka iyo dhaqdhaqaaqa beta-galactosidase ee muunado noole oo kala duwan, sida dhaqamada bakteeriyada ama lysates unug.Soo saarista o-nitrophenol, oo leh midab huruud ah, ayaa si fudud loo cabbiri karaa iyadoo la isticmaalayo spectrophotometer.

Daraasadaha muujinta hidda-wadaha: ONPG waxaa badanaa loo adeegsadaa cilmi-baarista bayoolajiga molecular si loo barto muujinta hiddaha.Marka la isku daro dhiirigeliyaha hiddo-wadaha xiisaha leh hidde-sidaha beta-galactosidase, cilmi-baarayaashu waxay cabbiri karaan waxqabadka dhiirrigeliyaha iyaga oo ku daraya ONPG oo cabbiraya soosaarka o-nitrophenol ee ka dhashay.Habkan, oo loo yaqaan baaritaanka wariyaha beta-galactosidase, wuxuu bixiyaa macluumaadka ku saabsan waxqabadka qoraalka hidda-wadaha.

Aqoonsiga bakteeriyada: Bakteeriyada qaar ayaa soo saarta beta-galactosidase, halka kuwo kalena aanay samayn.ONPG waxaa loo isticmaali karaa in lagu daro baaritaanada kale ee biochemical si loo aqoonsado noocyada bakteeriyada iyaddoo lagu salaynayo awoodooda ay ku nadiifiyaan ONPG.Habkan waxaa sida caadiga ah loo isticmaalaa baadhista bukaan-socodka iyo shaybaarrada microbiology.

Baadhitaanka enzyme-ka-horjoogayaasha ama firfircoonayaasha: ONPG waxaa loo isticmaali karaa in lagu baaro xeryahooda wax ka beddelaya waxqabadka beta-galactosidase.Marka la cabbiro dhaqdhaqaaqa enzyme ee joogitaanka xeryahooda kala duwan, cilmi-baarayaashu waxay aqoonsan karaan horjoogayaasha ama hawl-wadeennada kuwaas oo si dheeraad ah loogu baari karo awooddooda daweynta.