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X-GAL CAS: 7240-90-6 Qiimaha soo saaraha

5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) waa substrate chromogenic ah oo caadi ah oo loo isticmaalo bayoolaji molecular iyo codsiyada biochemistry.Waxaa si weyn loo isticmaalaa in lagu ogaado hidda-wadaha lacZ, kaas oo qeexaya enzyme β-galactosidase.


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Codsiga iyo Saamaynta

Beddelka Midabka: X-Gal caadi ahaan midab ma laha, laakiin, marka hydrolysis by β-galactosidase, waxay noqotaa buluug.Isbeddelka midabkani wuxuu u oggolaanayaa in la ogaado muuqaalka iyo qiyaasta waxqabadka β-galactosidase.

Helitaanka Hiddaha LacZ: X-Gal waxaa loo isticmaalaa in lagu aqoonsado unugyada ama dhismooyinka hidde-sidaha ee muujinaya hidda-wadaha lacZ.LacZ waxaa caadi ahaan loo adeegsadaa hidda-wadaha wariyaha ee bayooloojiyada molecular si loo qiimeeyo muujinta hidde-sidaha ama daraasadda waxqabadka dhiirrigeliyaha.

Baadhitaanka Gumeysiga: X-Gal waxaa badanaa loo isticmaalaa baaritaanka baarista gumeysiga bakteeriyada.LacZ-muujinta kolonyada bakteeriyada waxay u muuqdaan buluug marka ay ku koraan agar ka kooban X-Gal, taas oo sahlaysa in si sahlan loo aqoonsado iyo xulashada lacZ-positive colonies.

Falanqaynta Fusion Hida: X-Gal sidoo kale waxaa lagu isticmaalaa tijaabooyinka isku-dhafka hidda-wadaha.Marka hiddo-wade bartilmaameedku ku xidhan yahay hidda-wadaha lacZ, midabaynta X-Gal waxay muujin kartaa qaabka borotiinka fiyuuska ee unugga ama unugga.

Deganaanshaha Borootiinka: Midabaynta X-Gal waxaa loo isticmaali karaa in lagu baaro meelaynta borotiinka unugyada unugyada.Marka la isku daro borotiinka xiisaha u leh hidda-wadaha lacZ, hawsha β-galactosidase waxay tilmaami kartaa halka borotiinku ku sugan yahay unugga dhexdiisa.

X-Gal Analogues: Noocyada X-Gal ee la beddelay, sida Bluo-Gal ama Red-Gal, ayaa la sameeyay si loogu oggolaado nidaamyada horumarinta midabka beddelka ah.Analoogyadani waxay awood u siinayaan kala soocida unugyada lacZ-positive iyo lacZ-negative unugyada ama unugyada iyadoo la isticmaalayo midabyo kala duwan.

Tusaalaha Alaabta

7240-90-6-1
7240-90-6-2

Xiritaanka alaabta:

6892-68-8-3

Xog dheeraad ah:

Halabuurka C14H15BrClNO6
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CAS Maya. 7240-90-6
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