Tris (hydroxymethyl) nitromethane CAS: 126-11-4

Tris (hydroxymethyl) nitromethane, oo inta badan loo yaqaan Tris ama THN, waa iskudhis kiimiko oo leh caanaha molecular C4H11NO4.Waa crystalline jaale cirro leh oo aad ugu milmay biyaha.Tris waxaa si weyn loogu isticmaalaa sidii wakiilka bayoolajiga ee codsiyada bayoolajiga biochemical iyo molecular.Waxay ka caawisaa ilaalinta kala duwanaanta pH ee xalalka, taasoo ka dhigaysa mid qiimo leh farsamooyin kala duwan sida DNA iyo RNA go'doominta, PCR, gel electrophoresis, nadiifinta borotiinka, dhaqanka unugyada, kimistariga borotiinka, enzymology, iyo qiimeynta biochemical.Guryaha kaydinta Tris waxay u oggolaanayaan xaaladaha ugu wanaagsan tijaabooyinkan, hubinta natiijooyin sax ah oo la isku halayn karo.

Dalbo ama Codso Xigasho

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Codsiga iyo Saamaynta

Saamaynta:

Awoodda Buffering: Tris waxay u shaqeysaa sidii wakiilka wax-ku-oolka ah ee wax-ku-oolka ah sababtoo ah awoodda ay u leedahay inay aqbasho ama ku deeqdo borotoonnada, iyadoo ilaalinaysa kala duwanaanta pH ee xalalka.Waxa caadi ahaan loo isticmaalaa sidii qayb aasaasiga ah ee nidaamyada kaydinta si loo dejiyo pH ee muunadaha bayoolojiga iyo falcelinta.

Codsiyada:

Biology-ga Molecular: Tris waxaa si weyn loogu isticmaalaa sidii wakiilka bayooloji ee kala duwan ee farsamooyinka bayoolajiga molecular, oo ay ku jiraan DNA iyo RNA go'doomin, PCR, jel electrophoresis, iyo nadiifinta borotiinka.Waxay gacan ka geysataa ilaalinta jawiga pH deggan, taas oo u oggolaanaysa xaaladaha ugu habboon falcelinta enzymatic iyo isdhexgalka molecular.

Dhaqanka Unugyada: Tris waxaa inta badan loo adeegsadaa warbaahinta dhaqanka unugga si loo ilaaliyo pH joogto ah iyo dheelitirnaanta osmotic, taageerta korriinka unugyada caafimaadka qaba iyo jiritaanka.

Chemistry Protein: Tris waxa loo isticmaalaa tijaabooyinka kimistari borotiinka, sida milmaynta borotiinka, qiimaynta xasiloonida borotiinka, iyo daraasadaha xidhitaanka borotiinka-ligand.Waxay ka caawisaa in la ilaaliyo qiyaasta pH ee la rabo, hubinta isku laabma borotiinka saxda ah iyo dhaqdhaqaaqa.

Enzymology: Tris waxaa lagu shaqeeyaa baaritaanno enzymatic oo kala duwan si loo wanaajiyo xaaladaha pH ee looga baahan yahay dhaqdhaqaaqa enzymatic.Waxay hubisaa natiijooyin la isku halayn karo oo la soo saari karo, taasoo awood u siinaysa cabbirka saxda ah ee kinetics enzyme iyo daraasadaha xannibaadda.

Qiimaynta biochemical: Tris waxa loo isticmaalaa qayb ahaan baadhisyo badan oo kiimiko ah oo ay ugu wacan tahay sifooyinkeeda.Waxay ilaalisaa pH joogto ah inta lagu jiro midabaynta midabka, spectrophotometric, iyo enzymatic assays, kor u qaadida saxnaanta iyo isku halaynta natiijooyinka.