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TRIS-Acetate CAS: 6850-28-8 Qiimaha soo saaraha

TRIS-Acetate, waa kayd badiyaa loo isticmaalo tijaabooyinka bayooloji iyo bayooloji.Waa isku-darka saldhigga Tris iyo acetic acid, taasoo keentay xal pH-stable ah oo loo isticmaalo in lagu xakameeyo laguna ilaaliyo qiyaasta pH ee la rabo ee codsiyada kala duwan. TRIS-Acetate waxay si gaar ah faa'iido u leedahay daraasadaha DNA iyo RNA, maadaama ay bixiso jawi ku habboon hawlaha enzyme, electrophoresis, iyo jel electrophoresis.Waxay ka caawisaa ilaalinta xasiloonida iyo daacadnimada asiidhyada nucleicada inta lagu jiro hababka kala duwan, sida taxanaha DNA, falcelinta silsiladda polymerase (PCR), iyo agarose gel electrophoresis. Marka lagu daro cilmi baarista nucleic acid, TRIS-Acetate waxaa sidoo kale loo isticmaalaa go'doominta borotiinka iyo hababka nadiifinta , Soo saarista borotiinka xuubabka, iyo tijaabooyinka dhaqanka unugga.Awoodeeda wax-soo-saarka leh waxay ka dhigaysaa qalab qiimo leh meelo badan oo cilmi-baaris cilmiyeed ah, hubinta xaaladaha ugu fiican ee falcelinta noolaha iyo ilaalinta xasilloonida enzymes iyo borotiinnada.


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Codsiga iyo Saamaynta

Tris-acetate (TRIS-Acetate) waa kayd caadi ahaan loo isticmaalo tijaabooyinka bayoolojiga iyo bayoolojiga.Waxay ka kooban tahay isku darka tris (hydroxymethyl)aminomethane (Tris) iyo acetic acid, taas oo u shaqeysa sidii pH nidaamiye iyo xasiliye.pH ee kaydka TRIS-Acetate wuxuu caadi ahaan u dhexeeyaa 7.4 ilaa 8.4.
Saamaynta ugu weyn ee TRIS-Acetate waa in la joogteeyo pH deggan, taas oo muhiim u ah falcelinno badan oo bayooloji iyo bayooloji ah.Waxay u shaqeysaa sidii kayd iyadoo la yareynayo isbeddel kasta oo la taaban karo ee pH ee laga yaabo inay ku dhacaan asiidhyada ama saldhigyada lagu daray inta lagu jiro hababka tijaabada ah.
TRIS-Acetate waxay ka heshaa codsiyo kala duwan oo ku saabsan bayoolaji molecular, biochemistry, iyo bayoloji:
DNA iyo RNA Electrophoresis: TRIS-Acetate waxaa caadi ahaan loo isticmaalaa sida kaydiyaha agarose iyo polyacrylamide gel electrophoresis.Waxay bixisaa jawi pH deggan inta lagu jiro kala-soocidda DNA iyo jajabyada RNA oo ku salaysan cabbirkooda.
Falanqaynta borotiinka: TRIS-Acetate buffers waxaa loo isticmaalaa borotiinka electrophoresis, sida SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).Waxay hubisaa xasilloonida borotiinka iyo kala-soocidda inta lagu jiro habka.
Dareen-celinta Enzyme: Badbaadiyeyaasha TRIS-Acetate ayaa inta badan loo isticmaalaa imtixaannada enzymes iyo daraasadaha.Waxay siisaa kala duwanaanshaha pH-ga ugu fiican ee falcelinta enzymatic ee kala duwan waxayna ka caawisaa ilaalinta dhaqdhaqaaqa enzyme.
Dhaqanka Unugyada iyo Unugyada: TRIS-Acetate buffers waxaa loo isticmaalaa warbaahinta dhaqanka unugga si loo ilaaliyo pH ku habboon koritaanka unugyada iyo fidinta.Waxay ka caawisaa in la ilaaliyo xaaladaha jireed ee looga baahan yahay jiritaanka unugyada.

Xiritaanka alaabta:

6892-68-8-3

Xog dheeraad ah:

Halabuurka C6H15NO5
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CAS Maya. 6850-28-8
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