piperazine-1,4-bis(2-ethanesulfonic acid) cusbada disodium CAS:76836-02-7

Saamaynta:

Guryaha kaydinta: PIPES waxaa loo isticmaali karaa in lagu ilaaliyo heerarka pH joogto ah gudaha xad gaar ah, maadaama ay wax ku ool u tahay kaydinta qiyaasta pH-ga jireed ee 6.1-7.5.Tani waxay ka dhigaysaa mid waxtar u leh tijaabooyinka bayoolojiga ee kala duwan halkaasoo xakamaynta pH ay muhiim tahay.

Xasilooni: PIPES waxay ku xasilloon tahay heerkul kala duwan, taasoo ka dhigaysa mid ku habboon in loo isticmaalo tijaabooyinka lagu sameeyay xaalado kala duwan.

Codsiyada:

Dhaqanka unugga: PIPES waxa loo isticmaali karaa sidii hab-dhis u ah farsamooyinka dhaqanka unugga, sida ilaalinta pH-ga warbaahinta ama kaydinta loo isticmaalo koritaanka iyo dayactirka unugyada.

Daraasadaha borotiinka iyo ensaymka: PIPES waxaa caadi ahaan loo isticmaalaa daraasadaha borotiinka iyo enzyme si loo ilaaliyo pH xasilloon inta lagu jiro falcelinno kala duwan, gaar ahaan kuwa ku lug leh enzymes xasaasi ah ama borotiinno laga yaabo inay saameeyaan isbeddelada pH.

Electrophoresis: PIPES waxaa loo isticmaali karaa sida kaydinta codsiyada gel electrophoresis, ka caawinta in la ilaaliyo xaaladaha pH ee ugu fiican ee DNA ama kala soocida borotiinka.

Farsamooyinka bayoolajiga molecular: PIPES waxaa loo isticmaali karaa sidii kaydka farsamooyinka bayoolaji ee kala duwan, oo ay ku jiraan soo saarista DNA/RNA, PCR, iyo taxanaha DNA, hubinta natiijooyinka saxda ah iyadoo la ilaalinayo xaaladaha pH ee deggan.