Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside waxaa badanaa loo isticmaalaa substrate-ka enzyme-ka β-galactosidase. Marka β-galactosidase uu ku dhaqmo, isku-darka ayaa la hawo-siiyaa, taasoo keenta soo saarista p-nitrophenol ama o-nitrophenol, kuwaas oo leh midab jaalle ah oo si fudud loo ogaan karo. Tani waxay ka dhigaysaa mid waxtar u leh substrate-ka baaritaannada cabbira dhaqdhaqaaqa β-galactosidase.

Hal codsi oo caadi ah oo Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ah ayaa lagu sameeyaa baaritaannada hidda-wadaha ee wariyaha. Baaritaannadan, hidda-wadaha xiisaha leh waxaa lagu xiraa hidda-wadaha wariyaha, sida hidda-wadaha beta-galactosidase. Dhaqdhaqaaqa hidda-wadaha wariyaha waxaa markaa la kormeeraa iyadoo la cabbirayo dhaqdhaqaaqa β-galactosidase iyadoo la adeegsanayo Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside oo ah substrate-ka. Tani waxay u oggolaanaysaa cilmi-baarayaasha inay bartaan muujinta hidda-wadaha iyo nidaaminta.

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ayaa sidoo kale loo isticmaali karaa in lagu baaro daawooyinka ka hortaga ama firfircoonida β-galactosidase. Iyadoo la tijaabinayo iskudhisyo kala duwan ama musharixiinta daroogada, cilmi-baarayaashu waxay aqoonsan karaan molecules-ka wax ka beddelaya dhaqdhaqaaqa ensaymkan, kaas oo yeelan kara codsiyo daaweyn ah oo ku saabsan cuduro ama xaalado gaar ah.

Intaa waxaa dheer, isku-darkaan waxaa loo isticmaali karaa nadiifinta borotiinka. β-galactosidase waxaa badanaa lagu daraa borotiinnada xiisaha leh si loo fududeeyo ogaanshaha iyo nadiifinta. Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside waxaa loo isticmaali karaa substrate ahaan si loo baaro loona nadiifiyo borotiinnada isku-dhafka ah iyadoo la ogaanayo dhaqdhaqaaqa β-galactosidase.