ONPG CAS: 369-07-3 Qiimaha soo saaraha

Saamaynta ONPG sida substrate waa in lagu kala gooyaa enzyme β-galactosidase, taasoo keentay in la sii daayo badeeco jaalaha ah, o-nitrophenol.Midabkan isbeddelka ah waxaa lagu qiyaasi karaa spectrophotometrically, taas oo u oggolaanaysa qiyaasta waxqabadka β-galactosidase. Codsiga ONPG wuxuu ugu horreyntii ku jiraa qiimeynta muujinta hidda-socodka ee bayoolajiga molecular iyo cilmi-baarista microbiology.Waxaa caadi ahaan loo isticmaalaa in lagu cabbiro dhaqdhaqaaqa β-galactosidase sida wariye u ah daraasadaha muujinta hidda-wadaha, gaar ahaan bakteeriyada sida E. coli.Hidde-sidaha lacZ, kaas oo qeexaya β-galactosidase, ayaa inta badan loo isticmaalaa calaamad u ah falanqaynta muujinta hidda-wadaha, maadaama ay muujin karto xaalado gaar ah ama ay xakameyn karaan dhiirrigeliyeyaal gaar ah. Baaritaanka ONPG wuxuu bixiyaa hab ku habboon oo lagu kalsoonaan karo si loo qiimeeyo heerka muujinta hidde-sidaha iyadoo la cabbirayo waxqabadka β-galactosidase.Qiimayntan ayaa si weyn loogu isticmaalaa codsiyo kala duwan, sida barashada dhaqdhaqaaqa dhiirrigeliyaha, habaynta hidda-wadaha, iyo isdhexgalka borotiinka-proteinnada.Intaa waxaa dheer, waxaa loo isticmaali karaa in lagu go'aamiyo kinetics enzyme iyo in la qiimeeyo saameynta isbeddellada ama daaweynta dhaqdhaqaaqa enzyme.