Waxaa nagu hareereysan geesiyaal badan oo aan gabay, kuwaas oo aad mooddo inay caadi yihiin, laakiin run ahaantii aamusnaan wax badan ayay nagu soo kordhiyeen.Proteinase K waa "halyeyga aan la maqlin" ee warshadaha baarista unugyada, in kasta oo marka la barbar dhigo "weyn iyo xoogga" warshadaha, proteinase K waa fure aad u hooseeya oo aan muddo dheer iska indho-tiray muhiimaddeeda.Markii uu dillaacay cudurka faafa ee taajka cusub, baahida loo qabo borotiinka K ayaa kor u kacday, sahayda gudaha iyo dibedda ayaa aad uga dambaysa isticmaalka, qof walbana wuxuu si lama filaan ah u ogaaday in borotiinka K uu aad muhiim u yahay.
Waa maxay isticmaalka proteinase K?
Proteinase K waa serine protease leh firfircoonida enzyme proteolytic waxayna sii wadi kartaa dhaqdhaqaaqa meelo badan oo kala duwan (pH (4-12.5), kaydka cusbada sare, heerkulka sare ee 70 ° C, iwm.).Intaa waxaa dheer, waxqabadka proteinase K ma joojinayo SDS, HOL, EDTA, guanidine hydrochloride, guanidine isothiocyanate, iwm. ), cuntada (jilicsanaanta hilibka), maqaarka (timaha jilicsan), khamriga samaynta (caddaynta khamriga), diyaarinta amino acid (baalasha hoos u dhacay), soo saarista aashitada nucleic, isku-dhafka goobta, iwm., proteinase K Waxaa jira codsiyo.Codsiga ugu badan ee la isticmaalo waa soo saarista nucleic acid.
Proteinase K wuxuu enzymolyze karaa dhammaan noocyada borotiinada ku jira muunada, oo ay ku jiraan kuwa histones si adag ugu xidhan asiidhyada nucleic-ka, si ay asiidhka nucleic-ka ah laga sii daayo muunadda oo lagu sii daayo soosaarka, fududaynta tallaabada xigta ee soo saarista iyo nadiifinta.Marka la ogaado fayras nucleic acid, proteinase K waa mid ka mid ah qaybaha muhiimka ah ee xalka muunada fayraska.Proteinase K wuxuu dillaaci karaa oo kicin karaa borotiinka jaakad ee fayraska, kaas oo ka badbaado badan inta lagu jiro marxaladda gaadiidka iyo ogaanshaha;Intaa waxaa dheer, proteinase K wuxuu kaloo hoos u dhigi karaa RNase wuxuu ka hortagayaa hoos u dhaca fayraska RNA wuxuuna fududeeyaa ogaanshaha acid nucleic.
Caannimada habeen dhaxda ee proteinase K
Haddi ay noqoto dhinaca cilmi baadhista sayniska ama goobta IVD, soo saarista aashitada nucleic ayaa ah tijaabada ugu aasaasiga ah, sidaa darteed proteinase K waxa uu had iyo jeer ahaa jiritaan aad muhiim u ah.Si kastaba ha noqotee, waagii hore, borotiinka K wuxuu ahaa mid aad u caan ah marka loo eego doorkiisa.Qayb weyn oo ka mid ah tani waxay ahayd sababtoo ah saadka iyo xidhiidhka baahida borotiinka K ayaa ahaa mid aad u deggan.Dad yar ayaa u maleynaya in sahayda borotiinka K ay tahay dhibaato.
Markii uu dillaacay cudurka faafa ee taajka cusub, baahida loo qabo baaritaanka aashitada nukliyeerka ayaa kor u kacday.Dabayaaqadii Juun 2020, Shiinuhu waxa uu dhammaystiray ku dhawaad 90 milyan oo tijaabo taaj oo cusub ah, tiradanina waa ka sii cabsi badan marka loo eego heerka caalamiga ah.Tijaabooyinka soo saarista aashitada nucleic-ka, xoogga shaqada ee proteinase K waa qiyaastii 50-200 μg/mL.Guud ahaan, waxay qaadataa qiyaastii 100 μg oo borotiin ah K si loo soo saaro muunad ah acid nucleic ah.Isticmaalka dhabta ah, si loo kordhiyo waxtarka soo saarista nucleic acid, inta badan Proteinase K waxaa loo isticmaali doonaa qadar kordhay.Ogaanshaha aashitada nukliyeerka ee coronavirus-ka cusub wuxuu keenay xaddi badan oo borotiin ah baahida K.Sahayda asalka ah iyo dheelitirka baahida borotiinka K si dhakhso ah ayaa loo jabay, proteinase K wuxuu noqday wax muhiim ah oo ka hortagga faafitaanka habeenkii.
Dhibaatooyinka soo saarista proteinase K
Inkasta oo horumarinta cudurka faafa, qiimaha muhiimka ah ee borotiinka K ayaa lagu qiimeeyay dadku, waa wax laga xishoodo in sababtoo ah furaha-hoose ee borotiinka K, dhowr shirkadood oo gudaha ah ayaa ku lug lahaa soo saarista borotiinka K. Marka dadku Waxay rabaan inay abuuraan proteinase K wax soo saarka Intii lagu jiray habka wax soo saarka, waxaa la ogaaday in borotiinka K uu yahay borotiin gaar ah.Aad bay u dhib badan tahay in la ballaariyo awoodda wax-soo-saarka proteinase K muddo gaaban gudaheed.
Soosaarka baaxadda leh ee borotiinka K wuxuu wajahayaa dhibaatooyinka soo socda
1. Hadal hoose
Proteinase K wuxuu si aan gaar ahayn hoos ugu dhigi karaa borotiinnada badankood wuxuuna u keeni karaa sun halis ah unugga martida loo yahay.Sidaa darteed, heerka muujinta borotiinka K guud ahaan waa mid aad u hooseeya.Baaritaanka hababka muujinta iyo nuucyada aadka u muujinaya proteinase K guud ahaan waxay u baahan tahay wareeg dheer.
2. Hadhaaga midabka iyo asiidhyada nucleic-ka
Halsano baaxad weyn ayaa soo bandhigaysa xaddi badan oo midab leh iyo hadhaaga aashitada nucleic-ka ah.Way adag tahay in wasakhdaas laga saaro habka nadiifinta fudud, iyo nadiifinta adag waxay kordhisaa kharashka waxayna yaraynaysaa heerka soo kabashada.
3. Xasilooni darro
Proteinase K ma ahan mid deggan oo ku filan, waxay awood u yeelan kartaa enzymolyze lafteeda, wayna adagtahay in lagu kaydiyo si deggan 37 ° C muddo dheer iyada oo aan lahayn wakiil difaac.
4. Si fudud loo soo dajin karo
Markaad diyaarinayso budada la qalajiyey ee borotiinka K, si loo hubiyo in maadada adag ee proteinase K ee budada la qaboojiyey ay weyn tahay, waxaa lagama maarmaan ah in lagu daro wakiilka difaaca ee la qalajiyey ee xoogga badan, laakiin marka Isku darka borotiinka K wuxuu gaaraa 20mg/mL iyo wixii ka sareeya, waa sahlan tahay Isku geynta waxay sameysaa roobab, taas oo keeneysa dhibaatooyin weyn qallajinta borotiinka K oo leh maaddo adag.
5. Maalgelin ballaaran
Proteinase K waxa uu leeyahay firfircooni borotiin ah oo xoog leh waxana uu hydrolyze karaa borotiinada kale ee shaybaarka.Sidaa darteed, proteinase K wuxuu u baahan yahay meelo wax soo saar gaar ah, qalab, iyo shaqaale cilmi baaris iyo horumarin iyo wax soo saar.
XD BIOCHEM's proteinase K
XD BIOCHEM waxay leedahay muujinta borotiinka qaan-gaadhka ah iyo madal nadiifinta, waxayna leedahay khibrad qani ah oo ku saabsan muujinta iyo nadiifinta borotiinnada dib-u-habaynta iyo hagaajinta hababka wax-soo-saarka.Iyada oo loo marayo samaynta degdegga ah ee kooxda cilmi-baarista iyo horumarinta, habka wax-soo-saarka ballaaran ee proteinase K ayaa laga adkaaday.Wax soo saarka bilaha ah ee budada la qaboojiyey ayaa ka badan 30 KG.Alaabtu waxay leedahay waxqabad xasiloon, firfircooni gaar ah oo enzyme sare ah, mana laha hadhaaga cytochrome iyo nucleic acid.Ku soo dhawoow inaad la xidhiidho XD BIOCHEM Hel xidhmo tijaabo ah (E-mail:sales@xdbiochem.comTel: +86 513 81163739).
XD BIOCHEM xalal farsamo waxaa ka mid ah
Iyadoo la adeegsanayo isku-dhafka plasmid-ka koobiga badan, noocyada muujinta sare leh ee leh heerka muujinta 8g/L ayaa la doortay, taas oo ka gudubta dhibaatada heerka hoose ee borotiinka K.
Iyada oo la samaynayo habka nadiifinta tillaabooyin badan, cytochrome-ka martida loo yahay iyo hadhaaga aashitada nucleic ee proteinase K ayaa si guul leh looga saaray ka hooseeya qiimaha caadiga ah.
Iyada oo la adeegsanayo baadhis heersare ah oo lagu sameeyay qaabaynta kaydinta difaaca, kayd si adag u kaydin kara borotiinka K marka uu gaadho 37°C ayaa la doortay.
Xirmooyinka baarista waxay ka gudbaan dhibaatada ah in proteinase K ay fududahay in la isku geeyo oo lagu shubo xaddi aad u sarreeya, oo aasaaska u ah borotiinka K maadada adag ee qallajinta.
XD BIOCHEM proteinase K muunad
XD BIOCHEM proteinase K Tijaabada xasiloonida: ma jiri doono isbedel weyn oo ku yimaada dhaqdhaqaaqa kadib 80 d heerkulka qolka
XD BIOCHEM proteinase K Tijaabada xasiloonida: ma jiri doono isbeddel la taaban karo oo dhaqdhaqaaqa ka dib 80 d heerkulka qolka.
Isbarbardhigga saamaynta soo saarista aashitada nucleic ee XD BIOCHEM proteinase K iyo alaabada tartanka ku jirta.Habka soo saarista nucleic acid, XD BIOCHEM iyo borotiinka tartanka K ayaa loo isticmaalaa siday u kala horreeyaan.Waxtarka soo saarista XD BIOCHEM proteinase K waa ka sarreeyaa qiimaha Ct ee hidde-sidaha la beegsanayona wuu hooseeyaa.
Waqtiga boostada: Dec-31-2021