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MOPS sodium cusbo CAS: 71119-22-7
MOPS sodium cusbada, oo sidoo kale loo yaqaan3-(N-morpholino)propanesulfonic acid sodium cusbada, waa wakiilka kaydinta inta badan loo isticmaalo cilmi-baarista bayoolajiga iyo molecular.Waxaa loo isticmaalaa in lagu ilaaliyo kala duwanaanta pH-ga oo loo abuuro xaalado ku habboon falcelinta enzymatic, xasilloonida borotiinka, iyo koritaanka dhaqanka unugga.MOPS sodium cusbada ayaa si gaar ah wax ku ool u leh bixinta awoodda wax-ka-qabashada ee kala duwanaanta pH ee qiyaastii 6.5 ilaa 7.9.Waxaa si weyn loogu isticmaalaa hababka nadiifinta borotiinka, jel electrophoresis, daraasadaha enzyme, iyo tijaabooyinka dhaqanka unugyada.
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D-Glucuronic acid CAS:6556-12-3
D-Glucuronic acid waa asiidh sonkor ah oo laga helo gulukoos, waxaana si dabiici ah looga helaa jidhka bini'aadamka iyo unugyada kala duwan ee dhirta iyo xayawaanka.Waxay door muhiim ah ka ciyaartaa sun-saarista, ku xidhka iyo baabi'inta sunta iyo daawooyinka jidhka.Intaa waxaa dheer, D-Glucuronic acid waxay ku lug leedahay isku-darka iyo dheef-shiid kiimikaadka unugyo kala duwan, oo ay ku jiraan glycosaminoglycans, kuwaas oo muhiim u ah unugyada isku xiran.Waxay leedahay sifooyin antioxidant iyo faa'iidooyin caafimaad oo suurtagal ah, waxaana loo isticmaalaa kaabista cuntada iyo alaabta daryeelka maqaarka.
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2-Chloroethanesulfonic acid CAS:15484-44-3
2-Chloroethanesulfonic acid, oo sidoo kale loo yaqaan chloroethanesulfonic acid ama CES, waa iskudhis organic ah oo leh caanaha kiimikada C2H5ClSO3H.Waa dareere cad oo aan midab lahayn kaas oo aad ugu milmaya biyaha iyo dareeraha dabiiciga ah ee polar.
CES waxaa si weyn loogu isticmaalaa dhexdhexaadiye kiimiko oo kala duwan oo warshado kala duwan ah.Waxa ugu horrayn lagu shaqeeyaa isku-dhafka dawooyinka, kiimikooyinka beeraha, iyo midabaynta organic.Kooxdeeda sulfonic acid waxay ka dhigtaa reagent waxtar u leh soo bandhigida shaqeynta aashitada sulfonic molecules organic, kuwaas oo kor u qaadi kara milmayntooda, xasilloonida, ama noolaha.
Aysiidhkeeda xooggan awgeed, CES waxa kale oo loo isticmaali karaa kicin ama reagent aashito ah falcelinta dabiiciga ah.Dabeecaddeeda acidic waxay u oggolaaneysaa inay kor u qaaddo falcelinta sida esterifications, acylations, iyo sulfonations.Intaa waxaa dheer, waxay u adeegi kartaa sidii hagaajinta pH, wakiilka wax ilaalinaya, ama daxal-horjoogaha hababka warshadaha.
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PIPES monosodium cusbo CAS: 10010-67-0
Sodium hydrogen piperazine-1,4-diethanesulphonate, oo sidoo kale loo yaqaan HEPES-Na, waa wakiilka kaydinta inta badan loo isticmaalo cilmi-baarista bayooloji iyo bayooloji.Waxay ka caawisaa in la ilaaliyo kala duwanaanta pH ee 6.8 ilaa 8.2 ee codsiyada kala duwan, oo ay ku jiraan dhaqanka unugyada, imtixaannada enzymes, iyo farsamooyinka bayoolaji molecular.HEPES-Na waxay la jaan qaadaysaa nidaamyada noole ee kala duwan waxayna ku xasilloon tahay heerkul ballaaran oo kala duwan.
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4-Aminophenyl-β-D-galactopyranoside CAS:5094-33-7
4-Aminophenyl-β-D-galactopyranoside waa isku-dhismeed synthetic ah oo la mid ah substrate 3-Nitrophenyl-β-D-galactopyranoside (ONPG).Waxaa loo isticmaalaa sidii substrate-ka beta-galactosidase enzyme assays.Marka 4-aminophenyl-β-D-galactopyranoside waa hydrolyzed by beta-galactosidase, waxa ay sii daayaan xarun midab huruud ah oo loo yaqaan p-aminophenol.Dhaqdhaqaaqa beta-galactosidase waxaa lagu qiyaasi karaa iyada oo la qiyaaso qadarka p-aminophenol ee la soo saaro, sida caadiga ah iyada oo loo marayo baaritaanka midabka midabka ama spectrophotometric. , Xakamaynta enzyme ama firfircoonida, iyo aqoonsiga bakteeriyada.Awoodda lagu ogaanayo oo lagu cabbiro waxqabadka beta-galactosidase ayaa muhiim u ah dhinacyo badan oo cilmi baaris ah, oo ay ku jiraan bayoolajiga molecular, microbiology, iyo baarista caafimaad.
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3-(cyclohoxylamino)-2-hydroxy-1-propanesuhicic acid CAS:73463-39-5
3- (cyclohexylamino) -2-hydroxy-1-propanesuhicic acid waa iskudhis kiimiko oo leh caanaha kelli C12H23NO3S.Waxay ka tirsan tahay qoys ka kooban xeryahooda loo yaqaan asiidhyada sulfonic.Xaruntan gaarka ah waxay ka kooban tahay koox cyclohexylamino ah, koox hydroxy ah, iyo unug acid propanesuhicic ah.Waxaa loo isticmaalaa codsiyada warshadaha kala duwan, oo ay ku jiraan sida block dhismaha ee synthesis organic iyo sida reagent ah ee cilmi-baarista dawooyinka.Dhismaha gaarka ah ee xarunta iyo guryaha ayaa ka dhigaya mid ku habboon falcelinta kiimikaad ee gaarka ah iyo baaritaannada sayniska.
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HEIDA CAS: 93-62-9 Qiimaha soo saaraha
N- (2-Hydroxyethyl) iminodiacetic acid (HEIDA) waa isku dhaf kiimiko ah oo leh codsiyo badan oo dhinacyo kala duwan ah.Waa wax-ka-qabashada, taasoo la macno ah inay awood u leedahay inay ku xidho ion birta oo ay samayso dhismayaal deggan.
Kiimikada falanqaynta, HEIDA waxaa badanaa loo adeegsadaa sidii wakiil kakan marka la eego titrations iyo kala-goynta falanqaynta.Waxaa loo adeegsan karaa in lagu kala saaro ion birta, sida kalsiyum, magnesium, iyo birta, oo ka hortagto inay farageliyaan saxnaanta cabbirada gorfaynta.
HEIDA sidoo kale waxay ka heshaa codsiga warshadaha dawooyinka, gaar ahaan samaynta daawooyinka qaarkood.Waxaa loo isticmaali karaa sidii wakiil xasiliye iyo milmiye dawooyin liidata, taasoo gacan ka geysanaysa hagaajinta noolaha iyo waxtarkooda.
Meel kale oo loo isticmaalo HEIDA waa dhinaca daaweynta biyaha wasakhda ah iyo hagaajinta deegaanka.Waxaa loo shaqaaleysiin karaa sidii wakiil sequestering ah si looga saaro wasakhowga birta culus ee biyaha ama ciidda, si loo yareeyo suntooda korna loogu qaado dadaallada hagaajinta.
Intaa waxaa dheer, HEIDA waxaa loo adeegsaday isku dhafka isku-dubbaridka iyo qaab-dhismeedka birta-organic (MOFs), kuwaas oo leh codsiyo kala duwan oo ku saabsan kicinta, kaydinta gaaska, iyo dareenka.
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2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2
2-Nitrophenyl-beta-D-glucopyranoside waa kiimiko ka kooban molecule glucopyranoside oo ku xiran koox nitrofenyl ah.Waxaa caadi ahaan loo isticmaalaa sida substrate ee imtixaannada enzymatic si loo ogaado oo loo qiyaaso dhaqdhaqaaqa enzymes sida beta-glucosidase.Kooxda nitrophenyl waxaa lagu kala jeexjeexi karaa enzyme-ka, taasoo keentay in la sii daayo badeeco midab jaale ah oo lagu qiyaasi karo spectrophotometrically.Xaruntani waxay si gaar ah faa'iido u leedahay barashada kinetics-ka enzymka iyo baadhista sare u kaca ee ka hortagga enzyme-ka ama firfircoonayaasha.Waxa kale oo ay ka shaqeysaa cilmi-baarista biochemical ee baaritaanka dheef-shiid kiimikaadka karbohaydraytyada iyo sidii substrate-gaar ah-glycosidic-linkage.
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MES HEMISODIUM CAS: 117961-21-4
2-Amino-2-methyl-1,3-propanediol, oo sidoo kale loo yaqaan AMPD ama α-methyl serinol, waa unug kiimiko oo leh caanaha molecular C4H11NO2.Waa aalkolo amino ah oo sida caadiga ah loo isticmaalo dhexdhexaadinta kiimikaad ee isku dhafka daawooyinka iyo xeryahooda organic.AMPD waxay caan ku tahay awooda ay u leedahay inay u dhaqanto sidii caawiye chiral falcelinta asymmetric, taasoo ka dhigaysa mid qiimo leh soo saarista xeryahooda saafiga ah.Intaa waxaa dheer, waxa loo isticmaalay sidii qayb ka mid ah daryeelka shakhsi ahaaneed iyo alaabta la isku qurxiyo ee guryaha qoyaanka ah.
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Tris (hydroxymethyl) nitromethane CAS: 126-11-4
Tris (hydroxymethyl) nitromethane, oo inta badan loo yaqaan Tris ama THN, waa iskudhis kiimiko oo leh caanaha molecular C4H11NO4.Waa crystalline jaale cirro leh oo aad ugu milmay biyaha.Tris waxaa si weyn loogu isticmaalaa sidii wakiilka bayoolajiga ee codsiyada bayoolajiga biochemical iyo molecular.Waxay ka caawisaa ilaalinta kala duwanaanta pH ee xalalka, taasoo ka dhigaysa mid qiimo leh farsamooyin kala duwan sida DNA iyo RNA go'doominta, PCR, gel electrophoresis, nadiifinta borotiinka, dhaqanka unugyada, kimistariga borotiinka, enzymology, iyo qiimeynta biochemical.Guryaha kaydinta Tris waxay u oggolaanayaan xaaladaha ugu wanaagsan tijaabooyinkan, hubinta natiijooyin sax ah oo la isku halayn karo.
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FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9
Fluorescein mono-beta-D-galactopyranoside, oo sidoo kale loo yaqaan FMG, waa unug ka samaysan fluorescent oo caadi ahaan loo isticmaalo sidii substrate ee tijaabooyinka bayoolajiga unugyada kala duwan.Waxaa laga soo qaatay methyl-beta-D-galactopyranoside iyadoo lagu xirayo molecule fluorescein ah.FMG waxaa si weyn loo isticmaalaa in lagu barto dhaqdhaqaaqa beta-galactosidase, enzim kaas oo kicinaya hydrolysis ee lactose galay galactose iyo glucose.Adigoo isticmaalaya FMG substrate ahaan, cilmi-baarayaashu waxay la socon karaan dhaqdhaqaaqa enzymatic ee beta-galactosidase iyada oo loo marayo cabbirka qiiqa qiiqa.Hydrolysis of FMG by beta-galactosidase waxay keenaysaa in la sii daayo fluorescein, taasoo keentay in kororka signal fluorescent ah oo la qiyaasi karo. Xaruntan waxa kale oo loo isticmaalaa in lagu baaro aqoonsiga karbohaydrayt iyo isdhexgalka.FMG waxaa loo isticmaali karaa baaritaan molecular si loo daraaseeyo isku xirnaanta lectins-ka (borootiinnada si gaar ah ugu xira karbohaydraytyada) karbohaydraytyada ka kooban galactose.Isku-xidhka isku-xidhka FMG-lectin waa la ogaan karaa oo la qiyaasi karaa iyadoo lagu saleynayo isbeddelada qiiqa qiiqa. Guud ahaan, FMG waa qalab wax ku ool ah oo lagu baranayo dhaqdhaqaaqa enzyme iyo aqoonsiga karbohaydrayt, oo bixiya hab ku habboon oo xasaasi ah si loo cabbiro fluorescence loona qiimeeyo hababkan noolaha.
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3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7
3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPANAL, oo sidoo kale loo yaqaan NBD-aldehyde, waa xarun inta badan loo isticmaalo cilmi-baarista biochemistry iyo molecular bayooloji.
