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N, N-Bis (2-hydroxyethyl) -2-aminoethanesulfonic acid sodium cusbo CAS: 66992-27-6
N, N-Bis (2-hydroxyethyl) -2-aminoethanesulfonic acid sodium cusbo, oo sidoo kale loo yaqaan HEPES sodium cusbo, waa iskudhis kiimiko oo caadi ahaan loo isticmaalo wakiilka pH ee shaybaarada noolaha iyo kiimikaad.Waxay ka caawisaa ilaalinta kala duwanaanta pH xasilloon, taas oo ka dhigaysa mid ku habboon codsiyada sida dhaqanka unugyada, imtixaannada enzymes, daraasadaha borotiinka, electrophoresis, iyo hababka daawooyinka.HEPES sodium cusbada waxay hubisaa xaaladaha ugu fiican ee hababka noolaha waxayna kor u qaadaysaa saxnaanta iyo isku halaynta natiijooyinka tijaabada.
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S-Butyrylthiocholine iodide CAS: 1866-16-6
S-Butyrylthiocholine iodide waa unug kiimiko ah oo caadi ahaan loo isticmaalo baaritaanka biochemical iyo enzymatic.Waa substrate for butyrylcholinesterase enzyme (BChE) waxaana loo isticmaalaa in lagu cabbiro dhaqdhaqaaqiisa.
Marka S-Butyrylthiocholine iodide ay hydrolyzed by BChE, waxay soo saartaa thiocholine iyo butyric acid sida alaabta.Siideynta thiocholine waxaa lagu qiyaasi karaa iyadoo la isticmaalayo spectrophotometric ama fluorometric assay, taasoo u oggolaanaysa qiyaasidda dhaqdhaqaaqa BChE.
S-Butyrylthiocholine iodide waxaa inta badan loo isticmaalaa goobaha kiliinikada iyo cilmi baarista si loo qiimeeyo dhaqdhaqaaqa BhE ee shaybaarada sida balasmaha dhiigga ama unugyada.Waxaa loo isticmaali karaa in lagu qiimeeyo shaqada enzymatic ee BhE iyo doorka suurtagalka ah ee hababka noolaha ee kala duwan, iyo sidoo kale ogaanshaha iyo kormeerka xaaladaha caafimaadka qaarkood.
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ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium cusbo) CAS:30931-67-0
Diammonium 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate), oo inta badan loo yaqaan ABTS, waa substrate chromogenic ah oo caadi ahaan loo isticmaalo baaritaanka biochemical, gaar ahaan goobta enzymology.Waa iskudhis synthetic ah oo loo isticmaalo in lagu cabbiro dhaqdhaqaaqa enzymes kala duwan, oo ay ku jiraan peroxidases iyo oxidases.
ABTS waa mid aan midab lahayn qaabkeeda oksaydhka ah laakiin waxay isu beddeshaa buluug-cagaar marka ay oksaydheeyaan enzym iyadoo ay joogaan hydrogen peroxide ama ogsijiinta unugyada.Midabkan isbeddelka ah waxaa sabab u ah samaynta cation radical, kaas oo nuugaya iftiinka muuqaalka muuqda.
Falcelinta u dhaxaysa ABTS iyo ensaymku waxay soo saartaa badeeco midab leh oo lagu cabbiri karo spectrophotometrically.Xoojinta midabku waxay si toos ah ula mid tahay dhaqdhaqaaqa enzymatic, taas oo u oggolaanaysa cilmi-baarayaasha in ay qiyaas ahaan qiimeeyaan kinetics-ka enzyme-ka, xannibaadda enzyme-ka, ama isdhexgalka substrate-ka enzyme-ka.
ABTS waxay leedahay codsiyo balaaran oo dhinacyo kala duwan ah, oo ay ku jiraan baadhista kiliinikada, cilmi baarista dawooyinka, iyo sayniska cuntada.Aad bay xasaasi u tahay waxayna bixisaa kala duwanaansho firfircoon oo ballaaran, taasoo ka dhigaysa doorasho caan ah baaritaanno badan oo kiimiko ah.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
4-Nitrophenyl α-D-maltohexaoside waa iskudhis ka tirsan heerka isku xirka α-glycosidic.Waa nooc ka soo jeeda maltose, kaas oo ah disaccharide ka kooban laba unug oo gulukoos ah.Xaruntan, kooxda hydroxyl ee unugga gulukooska ugu horreeya waxaa lagu beddelaa unug nitrofenyl ah.
Xaruntan waxaa caadi ahaan loo isticmaalaa sida substrate ee imtixaannada enzymatic si loo barto dhaqdhaqaaqa enzymes kala duwan, gaar ahaan kuwa ku lug leh dheef-shiid kiimikaadka karbohaydraytyada.Kooxda nitrophenyl waxay u ogolaataa in si sahlan loo ogaado loona qiyaaso falcelinta enzymatic iyadoo la cabbirayo nuugista ama fluorescence ee sheyga dillaacsan.
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TUBEES CAS: 5625-37-6 Qiimaha soo saaraha
PIPES (piperazine-1,4-bisethanesulfonic acid) waa iskudhis buffering zwitterionic caadi ahaan loo isticmaalo cilmi bayooloji iyo bayooloji.Waa kaydiye wax ku ool ah oo leh awood sare oo lagu ilaalinayo xaaladaha pH ee xasilloon ee kala duwan pH ee 6.1 ilaa 7.5.PIPES waxay leedahay faragelinta ugu yar ee biomolecules waxayna ku habboon tahay qiyaasaha heerkulka ku-tiirsanaanta.Badanaa waxaa loo adeegsadaa farsamooyinka jel electrophoresis iyo samaynta dawooyinka sida wakiilka xasilinta.Guud ahaan, PIPES waa isku-dhafan oo si ballaaran loo isticmaalo goobo tijaabo oo kala duwan.
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3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
3,3′,5,5′-Tetramethylbenzidine, oo sidoo kale loo yaqaan TMB, waa isku dhaf kiimiko ah oo sida caadiga ah loo isticmaalo sida substrate-ka chromogenic ee baaritaanka difaaca jirka ee enzyme-kuxiran (ELISA) iyo baaritaanada kale ee biochemical.Waxaa badanaa loo isticmaalaa in lagu ogaado oo lagu qiyaaso joogitaanka enzymes sida horseradish peroxidase (HRP) ee muunado kala duwan oo bayooloji ah.TMB waxa ku dhaca isbeddel midab la'aan ah una beddela buluug iyadoo ay jiraan enzymes-yadan.Ka dib, falcelinta waxaa lagu joojin karaa iyadoo lagu darayo aashito taasoo u beddeleysa midabka buluuga ah midabka jaalaha ah ee kama dambaysta ah.Xoojinta midabka jaalaha ah waxay u dhigantaa qadarka enzyme ee jooga, taas oo u oggolaanaysa in la qiyaaso.
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APS-5 CAS: 193884-53-6 Qiimaha soo saaraha
(4-Chlorophenyl) thio-methanol 1-(dihydrogen phosphate) cusbada disodium (1:2) waa iskudhis kiimiko ah oo ka tirsan fasalka acridine derivatives.Waxay ka kooban tahay nidaamka giraanta 10-methylacridine oo leh koox thioether ah oo ku dheggan booska 4-chlorofenyl.Xarunta ayaa sidoo kale ka kooban koox methanol ah iyo laba kooxood oo fosfoortis ah kuwaas oo qayb ahaan laga saaray ion sodium.
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5-Bromo-4-chloro-3-indolyl fosfate disodium cusbo CAS:102185-33-1
5-Bromo-4-chloro-3-indolyl fosfate disodium cusbo (BCIP) waa iskudhis kiimiko ah oo caadi ahaan loo isticmaalo bayoolaji molecular iyo codsiyada biochemistry.Waa substrate chromogenic ah oo loogu talagalay enzymes phosphatase alkaline.
BCIP waxaa badanaa lala isticmaalaa nitroblue tetrazolium (NBT) substrate si loo ogaado dhaqdhaqaaqa fosfatase alkaline.Marka BCIP ay dephosphorylated by alkaline fosphatase, foomamka roobka buluugga ah, taas oo u oggolaanaysa muuqaalka joogitaanka enzyme-ka ama dhaqdhaqaaqa.
Xaruntani waxay si gaar ah faa'iido ugu leedahay codsiyada sida immunohistochemistry, isku-dhafka goobta, iyo baaritaanka immunosorbent-ku-xiran enzyme (ELISAs) si loo ogaado joogitaanka ama meelaynta biomolecules gaar ah ama acids nucleic ah.Da'da buluuga ah ee ay samaysay BCIP waxay bixisaa calaamad muuqata oo ka caawisa aqoonsiga iyo falanqaynta molecules bartilmaameedka ee muunadaha tijaabada ah.
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Sodium Salt Salt CAS: 139-41-3 Qiimaha soo saaraha
N, N-Bis (2-hydroxyethyl) glycine sodium cusbo waa iskudhis kiimiko ah oo loo isticmaalo sidii wakiilka kaydinta ee codsiyada kala duwan ee biochemical iyo biophysical.Waxay ka caawisaa ilaalinta heerka pH ee xaaladaha tijaabada ah, taas oo ka dhigaysa mid faa'iido u leh daraasadaha enzymes, cilmi-baarista borotiinka, dhaqanka unugyada, iyo farsamooyinka xoqidda galbeedka.
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4-Aminophthalhydrazide AMPPD CAS: 3682-14-2
4-Aminophthalhydrazide, oo sidoo kale loo yaqaan 4-APhH, waa iskudhis kiimiko oo leh caanaha molecular C8H8N2O.Waxay ka tirsan tahay fasalka xeryahooda hydrazide waxaana laga soo qaatay phthalic acid.
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N-Acetyl-L-cysteine CAS:616-91-1
N-Acetyl-L-cysteine (NAC) waa nooc la beddelay oo ah cysteine-ka amino acid.Waxay bixisaa isha cysteine-ka waxaana si sahal ah loogu beddeli karaa glutathione tripeptide, oo ah antioxidant awood leh oo jirka ku jira.NAC waxay caan ku tahay sifooyinka antioxidant iyo mucolytic, taas oo ka dhigaysa mid faa'iido u leh codsiyada caafimaadka ee kala duwan.
Sida antioxidant-ka, NAC waxay ka caawisaa inay unugyada ka ilaaliso waxyeelada ay keenaan xagjirrada xorta ah, noocyada ogsijiinta falcelinta, iyo sunta.Waxa kale oo ay taageertaa isku-dhafka glutathione, kaas oo door muhiim ah ka ciyaara hababka sun-saarista jidhka iyo ilaalinta nidaamka difaaca caafimaadka leh.
NAC waxaa lagu bartay faa'iidooyinka ay ka heli karto caafimaadka neefsashada, gaar ahaan shakhsiyaadka qaba xaaladaha sida boronkiitada raaga, COPD, iyo cystic fibrosis.Caadi ahaan waxaa loo isticmaalaa sidii rasiiye si ay u caawiso dhuuban iyo dabacsanaanta xabka, taas oo sahlaysa in la nadiifiyo marinnada hawo-mareenka.
Intaa waxaa dheer, NAC waxay muujisay ballanqaad ku aaddan taageeridda caafimaadka beerka iyada oo gacan ka geysanaysa ka saarista walxaha sunta ah, sida acetaminophen, xanuunka fudud ee caadiga ah.Waxa kale oo laga yaabaa inay yeelato saamayn ka hortag ah dhaawaca beerka ee ay keento cabbidda khamriga.
Marka laga soo tago sifooyinka ay leedahay antioxidant-ka iyo taageerada neefsashada, NAC waxaa lagu sahamiyay faa'iidooyinka suurtagalka ah ee caafimaadka dhimirka.Cilmi-baadhisyada qaarkood waxay soo jeedinayaan in laga yaabo inay saameyn togan ku yeelato cilladaha niyadda, sida niyad-jabka iyo xanuunka waswaaska (OCD).
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Acetylthiocholine iodide CAS: 1866-15-5
Acetylthiocholine iodide waa kiimiko kiimiko ah oo caadi ahaan loo isticmaalo sida substrate ee imtixaannada enzyme si loo cabbiro waxqabadka enzyme acetylcholinesterase (AChE).AChE waa enzim ka soo saara neurotransmitter acetylcholine, tallaabo muhiim ah oo lagu joojinayo gudbinta calaamadaha u dhexeeya unugyada dareemayaasha.
Marka acetylthiocholine iodide uu ku dhaqmo AChE, kooxda acetyl waa la saaraa, taasoo keentay samaynta thiocholine iyo acetate ions.Thiocholine ka dib waxay la falgashaa reagement aan midab lahayn oo loo yaqaan DTNB (5,5'-dithiobis(2-nitrobenzoic acid)) si ay u soo saarto iskudhis midab huruud ah oo loo yaqaan 5-thio-2-nitrobenzoate, kaas oo lagu qiyaasi karo spectrophotometrically.Heerka korriinka midabku wuxuu si toos ah ula siman yahay hawsha AChE ee muunadda.
