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5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide waa iskudhis loo isticmaalo daraasaadka biochemical ee kala duwan, gaar ahaan ogaanshaha iyo aragga ee hawlaha enzyme.Waa substrate-ka lagu nadiifin karo enzymes gaar ah, taasoo keentay in la sii daayo badeecad midab leh ama fluorescent ah.

Xaruntan waxaa caadi ahaan loo isticmaalaa baaritaannada si loo ogaado joogitaanka iyo dhaqdhaqaaqa enzymes sida beta-galactosidase iyo beta-glucuronidase.Enzymes-yadani waxay ka gooyaan kooxaha acetyl iyo glucosaminide substrate, taasoo horseedaysa samaynta koromophore buluug ama cagaar ah.

Qaab dhismeedka gaarka ah ee 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide waxay u oggolaaneysaa in si sahlan loo ogaado iyo qiyaasidda dhaqdhaqaaqa enzyme.Isticmaalkeeda farsamooyin tijaabo ah oo kala duwan, oo ay ku jiraan histochemistry, immunohistochemistry, iyo imtixaannada unugyada ku salaysan, ayaa gacan ka geystay faham wanaagsan oo ku saabsan hawlaha enzyme.


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Codsiga iyo Saamaynta

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) waa substrate chromogenic ah oo inta badan loo isticmaalo ogaanshaha beta-glucuronidase (GUS).GUS waa enzyme laga helo noole kala duwan, oo ay ku jiraan bakteeriyada, dhirta, iyo naasleyda.X-Gluc waxaa inta badan lagu isticmaalaa qiimeynta wariyaha GUS iyo cilmi-baarista bayoolajiga molecular.

Codsiga ugu weyn ee X-Gluc wuxuu ku jiraa farsamooyinka wasakhaynta histochemical, halkaas oo ay ku arki karto muujinta iyo waxqabadka enzyme GUS.Substrate-kani waa unug-mullaali kara wuxuuna noqdaa hydrolyzed by GUS, taasoo keentay samaynta roob da'o buluug ah ama badeeco aan milmi karin.Midabaynta buluuga ah waxay u ogolaataa cilmi-baarayaasha inay aqoonsadaan oo ay meeleeyaan dhaqdhaqaaqa GUS ee unugyada, unugyada, iyo noolaha oo dhan.

X-Gluc sidoo kale waxaa loo isticmaali karaa qiyaasaha tirada si loo cabbiro dhaqdhaqaaqa GUS enzyme.Xoogga midabka buluuga ah ama qadarka alaabta la sameeyay ayaa lala xiriirin karaa heerka muujinta GUS ama dhaqdhaqaaqa enzymatic.

Intaa waxaa dheer, X-Gluc waxaa lagu shaqaaleysiiyay cilmi-baarista hidde-sidaha dhirta si loo barto muujinta hidda-wadaha, waxqabadka dhiirrigeliye, iyo isbeddelka dhirta.Waxa kale oo loo isticmaalay nidaamyada bakteeriyada si loo xidho loona ogaado borotiinnada fiyuuska GUS.

Xiritaanka alaabta:

6892-68-8-3

Xog dheeraad ah:

Halabuurka C16H18BrClN2O6
Qiimayn 99%
Muuqashada budo cad
CAS Maya. 4264-82-8
Baakad Yar iyo weyn
Nolosha shelf 2 sano
Kaydinta Ku kaydi meel qabow oo qalalan
Shahaado ISO.

 


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