4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

Substrate Enzyme: pNAG waxaa si weyn loogu isticmaalaa sidii substrate gaar ah oo loogu talagalay enzymes kala duwan oo ku lug leh hydrolysis of β-D-glucoside bonds.Marka enzymes-yadani ay jebiyaan molecule pNAG, waxay sii daayaan p-nitrophenol.Tani waxay u oggolaanaysaa cilmi-baarayaasha inay cabbiraan oo ay qiyaasaan dhaqdhaqaaqa enzymatic.

Qiimaynta Dhaqdhaqaaqa Enzyme: Hydrolysis of pNAG by enzymes gaar ah ayaa lagu ogaan karaa oo lagu qiyaasi karaa spectrophotometrically.Tani waxay ka dhigaysaa pNAG mid ku habboon tijaabooyinka dhaqdhaqaaqa enzyme, halkaasoo qaddarka p-nitrophenol ee la soo saaray ay si toos ah ula mid tahay dhaqdhaqaaqa enzymatic.

Baadhitaanka Wax-soo-saarka Sare: pNAG waxaa caadi ahaan loo adeegsadaa qiimaynta baadhista-sare ee la soo saaray si loo aqoonsado oo loo garto ka-hortagga ensaymes ama hawl-wadeennada.Iyadoo la qiimeynayo saameynta xeryahooda kala duwan ee dhaqdhaqaaqa enzymatic, cilmi-baarayaashu waxay aqoonsan karaan musharaxiinta daroogada ama modulators ee shaqada enzyme.

Daraasaadka Muujinta Hida: pNAG sidoo kale waxaa loo isticmaalaa cilmi-baarista bayoolajiga molecular si loo barto muujinta hidda-wadaha iyo nidaaminta.Iyadoo la cabbirayo dhaqdhaqaaqa enzymatic ee enzymes gaar ah oo isticmaalaya pNAG sida substrate, cilmi-baarayaashu waxay baari karaan saameynta muujinta hidda-socodka ee shaqada enzymes iyo dhaqdhaqaaqa.