2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

2-Nitrophenyl-beta-D-glucopyranoside waa isku-dhis kiimiko ah oo ka kooban molecule glucopyranoside ah oo ku xiran kooxda nitrophenyl. Waxaa caadi ahaan loo isticmaalaa sidii substrate baaritaannada enzymatic si loo ogaado oo loo cabbiro dhaqdhaqaaqa ensaymada sida beta-glucosidase. Kooxda nitrophenyl waxaa kala jari kara ensaymka, taasoo keenta in la sii daayo badeecad midab jaalle ah oo lagu cabbiri karo spectrophotometric. Isku-darkani wuxuu si gaar ah waxtar ugu leeyahay barashada kinetics-ka ensaymka iyo baaritaanka wax-soo-saarka sare ee inhibitors-ka ama firfircoonayaasha ensaymka. Waxaa sidoo kale loo isticmaalaa cilmi-baarista bayoolaji ahaan baaritaanka dheef-shiid kiimikaadka karbohaydraytka iyo substrate-ka glycosidic-linkage-gaarka ah.

Dalbo ama Codso Qiimayn

Faahfaahinta Badeecada

Calaamadaha Alaabta

Codsiga iyo Saamaynta

Substrate-ka Ensaymka: ONPG waxaa badanaa loo isticmaalaa substrate-ka beta-galactosidase, ensaym kaas oo hydrolyzes ONPG si uu u soo saaro isku-dhis midab jaalle ah (o-nitrophenol) oo si fudud loogu ogaan karo spectrophotometry. Falcelintan ensaymikada waxaa loo isticmaali karaa in lagu cabbiro dhaqdhaqaaqa beta-galactosidase, taasoo ka dhigaysa ONPG qalab qiimo leh oo ku jira daraasadaha ensaymology-ga.

Tijaabooyinka bayoolajiga ee molecular-ka: ONPG waxaa loo isticmaalaa sidii substrate baaritaanno bayoolajiga ee molecular-ka oo kala duwan, gaar ahaan baaritaannada hiddaha ee weriyaha beta-galactosidase. Tijaabooyinkan, substrate ku salaysan ONPG ayaa loo isticmaalaa in lagu cabbiro dhaqdhaqaaqa hidda-wadaha weriyaha, kaas oo caadiyan lagu xakameeyo taxane gaar ah oo dhiirrigeliye ah oo xiiso leh. Waxqabadka beta-galactosidase, oo lagu muujiyey isbeddelka midabka ee laga soo saaro hydrolysis-ka ONPG, wuxuu bixiyaa macluumaad qiimo leh oo ku saabsan dhaqdhaqaaqa dhiirrigeliyaha.

Falanqaynta muujinta hidda-wadaha: ONPG waxaa sidoo kale loo isticmaalaa falanqaynta muujinta hidda-wadaha. Iyadoo la isku xirayo taxanaha xiisaha ee dhiirrigeliyaha iyo hidda-wadaha beta-galactosidase, cilmi-baarayaashu waxay cabbiri karaan dhaqdhaqaaqa beta-galactosidase iyagoo adeegsanaya ONPG oo ah substrate. Heerka dhaqdhaqaaqa beta-galactosidase wuxuu ka tarjumayaa xoogga iyo dhaqdhaqaaqa dhiirrigeliyaha, taasoo u oggolaanaysa qiimeynta heerarka muujinta hidda-wadaha.

Codsiyada ogaanshaha: ONPG waxaa loo isticmaali karaa codsiyada ogaanshaha, gaar ahaan ogaanshaha noolaha faafa. Bakteeriyada kala duwan, sida Escherichia coli iyo noocyada qaarkood ee Shigella iyo Salmonella, waxay soo saaraan beta-galactosidase kaasoo burburin kara ONPG. Falcelintan hydrolysis waxay soo saartaa isbeddel midab oo muuqda, kaas oo loo isticmaali karo in lagu go'aamiyo joogitaanka bakteeriyadan muunadaha kiliinikada.

Muunadda Badeecada

Baakaynta Alaabta:

Macluumaad Dheeraad ah:

Halabuurka	C12H15NO8
Baaritaan	99%
Muuqaalka	Budada jaalaha ah ee khafiifka ah
Lambarka CAS.	2816-24-2
Baakad	Yar iyo weyn
Muddada Khafiska	2 sano
Kaydinta	Ku kaydi meel qabow oo qalalan
Shahaadada	ISO.