2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

2-Nitrophenyl-beta-D-glucopyranoside waa kiimiko ka kooban molecule glucopyranoside oo ku xiran koox nitrofenyl ah.Waxaa caadi ahaan loo isticmaalaa sida substrate ee imtixaannada enzymatic si loo ogaado oo loo qiyaaso dhaqdhaqaaqa enzymes sida beta-glucosidase.Kooxda nitrophenyl waxaa lagu kala jeexjeexi karaa enzyme-ka, taasoo keentay in la sii daayo badeeco midab jaale ah oo lagu qiyaasi karo spectrophotometrically.Xaruntani waxay si gaar ah faa'iido u leedahay barashada kinetics-ka enzymka iyo baadhista sare u kaca ee ka hortagga enzyme-ka ama firfircoonayaasha.Waxa kale oo ay ka shaqeysaa cilmi-baarista biochemical ee baaritaanka dheef-shiid kiimikaadka karbohaydraytyada iyo sidii substrate-gaar ah-glycosidic-linkage.

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Substrate Enzyme: ONPG waxaa caadi ahaan loo isticmaalaa sidii substrate beta-galactosidase, enzym ka soo saara ONPG si ay u soo saarto iskudhis midab huruud ah (o-nitrophenol) kaas oo si fudud loo ogaan karo spectrophotometrically.Falcelintan enzymatic waxaa loo isticmaali karaa in lagu cabbiro dhaqdhaqaaqa beta-galactosidase, samaynta ONPG qalab qiimo leh oo ku jira daraasaadka enzymology.

Qiimaynta bayoolaji molecular: ONPG waxa loo istcimaalaa substrate ahaan qiimaynta bayoolaji ee kala duwan, gaar ahaan qiimaynta hidda-sidaha weriye beta-galactosidase.Qiimayntan, substrate-ku-salaysan ONPG ayaa loo isticmaalaa in lagu cabbiro dhaqdhaqaaqa hidda-wadaha wariyaha, kaas oo sida caadiga ah lagu maamulo xayaysiiye gaar ah oo xiisa leh.Hawsha beta-galactosidase, oo lagu muujiyay isbeddelka midabka ee lagu sameeyay ONPG hydrolysis, ayaa bixisa macluumaad qiimo leh oo ku saabsan waxqabadka dhiirrigeliyaha.

Falanqaynta muujinta hidda-wadaha: ONPG waxa kale oo loo adeegsadaa falanqaynta muujinta hiddaha.Iyadoo la isku xirayo taxanaha dhiirrigeliyaha ee xiisaha hidda-wadaha beta-galactosidase, cilmi-baarayaashu waxay cabbiri karaan waxqabadka beta-galactosidase iyagoo isticmaalaya ONPG substrate ahaan.Heerka dhaqdhaqaaqa beta-galactosidase wuxuu ka tarjumayaa xoogga iyo waxqabadka dhiirrigeliyaha, taas oo u oggolaanaysa qiimeynta heerarka muujinta hidda-socodka.

Codsiyada ogaanshaha: ONPG waxaa loo isticmaali karaa codsiyada ogaanshaha, gaar ahaan ogaanshaha noolaha pathogenic.Bakteeriyada kala duwan, sida Escherichia coli iyo noocyada qaarkood ee Shigella iyo Salmonella, waxay soo saaraan beta-galactosidase taasoo kala goyn karta ONPG.Falcelintan hydrolysis waxay soo saartaa isbeddel muuqda oo midab leh, kaas oo loo isticmaali karo si loo go'aamiyo joogitaanka bakteeriyadan shaybaarada caafimaadka.

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Xog dheeraad ah:

Halabuurka	C12H15NO8
Qiimayn	99%
Muuqashada	budo huruud ah oo khafiif ah
CAS Maya.	2816-24-2
Baakad	Yar iyo weyn
Nolosha shelf	2 sano
Kaydinta	Ku kaydi meel qabow oo qalalan
Shahaado	ISO.